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| Product Name | 384-Well qPCR Plate, White, FrameStar Design, Low-Profile |
| Catalog No. | IGM-HMM-0068 |
| Description | White 384-well qPCR plate with rigid polypropylene frame (FrameStar design) providing exceptional thermal stability and well-to-well uniformity. The white wells maximize fluorescence signal reflection for qPCR while the polypropylene frame prevents warping during thermal cycling up to 100 °C. Each well has an ultra-smooth interior surface to minimize sample and enzyme binding. Compatible with all major qPCR and thermal cycler platforms including ABI, Bio-Rad, Roche, and Qiagen instruments. |
| Intended Use | Real-time quantitative PCR (qPCR) and RT-qPCR; digital PCR sample preparation; high-throughput genotyping; gene expression analysis; mutation detection; pathogen detection assays in 384-well format. |
| Principle / Technology | White wells reflect fluorescence excitation/emission maximizing signal-to-noise ratio in qPCR; polypropylene frame is dimensionally stable across 4-100 °C thermal cycling range; low-profile well design (0.1 mL max volume) minimizes thermal mass for rapid temperature equilibration; ultra-smooth surface reduces protein and nucleic acid binding |
| Detection Method | Load 5-30 µL reaction volume per well; seal with optically clear adhesive film or heat seal; place in thermal cycler with appropriate 384-well block adapter |
| Sample Type | PCR reaction mix typically 5-20 µL per well; DNA/cDNA template; SYBR Green, TaqMan, or Molecular Beacon probe chemistries |
| Components / Formulation | Polypropylene frame with white polystyrene wells; 384 wells (16 rows × 24 columns) with alphanumeric grid marking; raised rim design for effective sealing; cut corner at A1 position for orientation |
| Storage Conditions | 15-30 °C in original packaging; protect from dust and contamination |
| Shelf Life | 36 months from manufacture date when stored properly |
| Package Specifications | 50 plates per case (10 sleeves of 5 plates each); individually or bulk available |
| Product Form | Rigid 384-well white plate with polypropylene frame; compatible with robotic handling systems |
| Key Features | FrameStar rigid frame prevents thermal warping; white wells maximize qPCR signal; low-profile 0.1 mL wells; alphanumeric well identification; raised rims for reliable sealing; DNase/RNase-free certified; PCR clean certified (no amplifiable human DNA); compatible with major qPCR instruments; SBS/ANSI footprint for robotic handling |
| Purity | DNase/RNase-free certified per lot; PCR clean — no amplifiable human or bacterial DNA detected; heavy metals not detected; endotoxin not applicable |
| Concentration | Not applicable — solid consumable product |
| Activity / Unit Definition | Performance verified per dimensional and functional specifications |
| Molecular Weight | Not applicable — polymer/glass consumable product |
| Source / Origin | Virgin polypropylene, polystyrene, borosilicate glass as specified |
| pH Range / Optimal pH | Not applicable — solid consumable |
| Shipping Conditions | Ambient; protect from crushing; pack flat to prevent warping |
| Expiration Date / Stability | 36 months; use within recommended shelf life for optimal sealing performance; older plates may require extended sealing time/temperature |
| Regulatory / Compliance | CE marked; manufactured under ISO 9001 and ISO 13485; For laboratory use; not classified as medical device (RUO) |
| Compatibility | Compatible with Applied Biosystems (7900HT, ViiA7, QuantStudio series), Bio-Rad (CFX384, CFX Opus 384), Roche LightCycler 480, Qiagen Rotor-Gene Q (with appropriate adapter), Eppendorf Mastercycler, and Analytik Jena qTOWER; verify block specifications for non-standard cyclers |
| Recommended Buffer System | Not applicable — use with standard aqueous buffers and organic solvents per chemical compatibility |
| Application Notes / Precautions | Single use only for sterile products; inspect packaging integrity before use; dispose per biohazard guidelines |
| Batch-to-Batch Consistency | Dimensional tolerance within specification; functional testing per AQL sampling plan |
For research use only, not for clinical use.
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