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| Product Name | 384-Well PCR Plate, Clear |
| Catalog No. | IGM-HMM-0044 |
| Description | Clear polypropylene 384-well PCR plate in standard high-density format for ultra-high-throughput thermal cycling applications. Miniaturized reaction volumes from 5 to 30 uL. Compatible with 384-well thermal cycler blocks. |
| Intended Use | High-throughput PCR screening, massively parallel genotyping, high-density qPCR arrays, and automated drug discovery assays requiring 384-well format thermal cycling. |
| Principle / Technology | Miniaturized well format in same ANSI/SLAS footprint as 96-well plate quadruples throughput density. Thin-walled polypropylene wells ensure efficient thermal transfer. Clear polymer allows visual inspection of reaction volumes. |
| Detection Method | Thermal gradient uniformity by IR thermography; evaporation rate over extended cycling; automated liquid handler compatibility testing |
| Sample Type | Genomic DNA for SNP panels, cDNA libraries for expression screening, synthetic DNA templates, microbial colony PCR templates |
| Performance Range / Specifications | Reaction volume 5-30 uL per well; well-to-well thermal variation <0.5 C; evaporation <5% over 40 cycles with proper sealing |
| Sensitivity / LOD | Minimum reliable detection at 5 uL reaction volume; no detectable well-to-well contamination in 384-plex format |
| Specificity | Free of PCR inhibitors; amplification efficiency 90-110% across all 384 wells in uniformity test |
| Reaction Conditions / Protocol | Dispense 10-20 uL reactions using automated liquid handler or multichannel pipette; seal with optical adhesive film; centrifuge; thermal cycle per protocol. |
| Components / Formulation | Medical-grade polypropylene (USP Class VI), alphanumeric well identification, cut corner A1 orientation |
| Storage Conditions | 15-30 C, clean dry environment; store in sealed packaging until use |
| Shelf Life | 24 months from manufacture |
| Package Specifications | 50 plates per case, sealed in blocks of 10; individually sealed PE bags |
| Product Form | 384-well plate, clear polypropylene, full-skirted or non-skirted options, individually sealed |
| Quality Control | Well dimensional inspection per modified ANSI/SLAS 2-2004; PCR performance test with 384-plex genotyping panel; nuclease and bioburden testing |
| Key Features | 384-well density for high-throughput screening; thin-walled for rapid thermal transfer; compatible with 384-channel pipettors; barcode-ready plate surface |
| Purity | DNase/RNase-free certified; PCR inhibitor-free; endotoxin <0.005 EU/well |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade polypropylene; precision injection molded under cleanroom conditions per ISO 13485 |
| pH Range / Optimal pH | Polypropylene inert across pH 1-14; optical clarity maintained across buffer pH range |
| Shipping Conditions | Ambient temperature; stack plates with protective interleaf sheets; use rigid outer carton |
| Expiration Date / Stability | 2 years from manufacture; PCR performance maintained throughout shelf life in sealed packaging |
| Regulatory / Compliance | ISO 13485:2016; CE marked; ANSI/SLAS compliant; FDA registered facility |
| Compatibility | Compatible with Applied Biosystems 384-well thermal cyclers, Bio-Rad CFX384, Roche LightCycler 480 multiwell plate 384, and other standard 384-well block instruments; automated liquid handler compatible |
| Recommended Buffer System | Compatible with all commercial PCR and qPCR master mixes; validated with SYBR Green, TaqMan, and multiplex assay buffers |
| Application Notes / Precautions | Due to small well volume, use electronic or positive-displacement pipetting for accuracy; ensure complete sealing to prevent cross-well evaporation; centrifuge at 2000 x g for 2 minutes after sealing |
| Batch-to-Batch Consistency | Well dimensions verified by laser profilometry per 12-cavity mold section; thermal cycling performance benchmarked against reference plate per batch |
For research use only, not for clinical use.
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