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| Product Name | 32-35 µm PMMA Butyl and Phenyl Hydrophobic Interaction Media Up to 30 mg/mL |
| Catalog No. | IGM-HMM-0285 |
| Description | Alta DiagnoTech hydrophobic interaction media use monodisperse, highly crosslinked PMMA microspheres with a hydrophilic surface and immobilized butyl or phenyl groups. High-salt binding and low-salt elution support protein polishing with low nonspecific adsorption, controlled backpressure and a mechanically stable packed bed. |
| Intended Use | Further separation and purification of biomolecules after ion-exchange chromatography. |
| Principle / Technology | Hydrophobic interaction chromatography with biomolecule adsorption at high salt concentration and sequential elution as salt concentration decreases. |
| Performance Range / Specifications | Four variants combine 32 or 35 µm particles with butyl or phenyl ligands and provide approximately 15-30 mg/mL dynamic binding capacity for lysozyme. |
| Storage Conditions | 20% ethanol at 4-25 °C |
| Package Specifications | 30 mL, 100 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L standard packs |
| Product Form | Hydrophobic interaction chromatography medium |
| Key Features | Monodisperse PMMA microspheres Butyl and phenyl ligand options Low nonspecific adsorption High mechanical strength and low backpressure |
| Batch-to-Batch Consistency | Monodisperse particles support stable batch performance. |
| Product Type | Hydrophobic interaction chromatography medium |
| Function / Efficacy | Hydrophobicity-based polishing and purification of proteins and other biomolecules. |
| Chromatography Mode | Hydrophobic interaction chromatography |
| Matrix | Hydrophilically modified, highly crosslinked poly(methyl methacrylate) (PMMA) |
| Particle Size | 32 or 35 µm, depending on variant |
| Ligand / Bonded Phase | Butyl or phenyl |
| Dynamic Binding Capacity | Approximately 23 or 15 mg/mL for the butyl variants and approximately 30 or 15 mg/mL for the phenyl variants, measured with lysozyme |
| Maximum Operating Pressure | 1.0 MPa |
| CIP / Sanitization Conditions | 1 M NaOH or 70% ethanol |
| Recommended Flow Rate | 50-300 cm/h |
| pH Stability | pH 2-12 |
| Chemical Stability | Compatible with common buffers, 1 M acetic acid, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine hydrochloride and 8 M urea; avoid strong oxidants. |
| Operating Temperature | 15-30 °C |
For research use only, not for clinical use.
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