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| Product Name | 293 Cell Serum-Free Culture Medium |
| Catalog No. | RESK-0483 |
| Description | 293 Cell Serum-Free Culture Medium is an animal-component-free, protein-free, fully chemically defined medium. It is suitable for large-scale serum-free suspension culture and subsequent transfection-based expression of HEK293 cell lines (such as HEK293F, HEK293T, HEK293H and other suspension-adapted HEK293 lines). The product is specifically optimized for the research, development and production of AAV and protein-related products, supporting high-density cell expansion and stable product expression, and offers broad adaptability across HEK293 cell types. To accommodate different culture systems and production processes, a matching feed medium is available, and the medium is also compatible with other commercial feed products. |
| Intended Use | Large-scale serum-free suspension culture and transfection-based expression of HEK293 cell lines, supporting AAV and protein product research, development and production. |
| Principle / Technology | Fully chemically defined, animal-component-free and protein-free formulation optimized for HEK293 suspension culture and transfection. |
| Sample Type | HEK293 cell lines (e.g., HEK293F, HEK293T, HEK293H, and other suspension-adapted HEK293 lines) |
| Performance Range / Specifications | 1. Animal-component-free, protein-free and fully chemically defined. 2. Suitable for high-density suspension culture of various HEK293 cell lines, with strong adaptability and rich nutrients. 3. Suitable for large-scale bioreactor culture processes, supporting batch, fed-batch and perfusion culture modes. 4. Effectively supports transfection and subsequent product expression, ensuring stable, high-quality viral titer expression. Batch culture of HEK293 cells achieves a PVCD above 1.0 x 10^7 cells/mL, and transfection efficiency reaches over 90%. |
| Reaction Conditions / Protocol | 1. Supports batch, fed-batch and perfusion culture modes. 2. If the medium has not been used for a long period (approximately 2 months), it is recommended to add 2 mM L-glutamine before use. 3. No FBS supplementation is required. Antibiotics are generally not required and may be added according to experimental needs. |
| Components / Formulation | Fully chemically defined; animal-component-free and protein-free. |
| Storage Conditions | Store at 2-8 °C. Protect from light. |
| Shelf Life | 1 year. |
| Package Specifications | 1000 mL |
| Product Form | Liquid medium |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 1 year when stored at 2-8 °C protected from light. If unused for approximately 2 months, add 2 mM L-glutamine before use. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Internally tested with suspension-adapted HEK293F and HEK293T cells; in principle applicable to other suspension-adapted 293 cells. A matching feed medium is available, and the medium is also compatible with other commercial feed products. |
| Application Notes / Precautions | 1. No FBS needs to be added during use. Antibiotics (penicillin-streptomycin) are generally unnecessary but may be added if aseptic handling is a concern. 2. For long-term stored medium, supplement with 2 mM L-glutamine before use. |
For research use only, not for clinical use.
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