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| Product Name | 10-30 µm High-Resolution PS-DVB Ion-Exchange Media with 2-8 MPa Tolerance |
| Catalog No. | IGM-HMM-0288 |
| Description | Alta DiagnoTech high-resolution ion-exchange media use highly crosslinked porous PS-DVB microspheres with a chemically stable hydrophilic surface. Six cation- and anion-exchange options combine 10, 15 or 30 µm particles with high resolution, low backpressure and strong physical stability. |
| Intended Use | Intermediate through ultra-fine purification of biological samples using strong cation- or anion-exchange chromatography. |
| Principle / Technology | Strong ion exchange using sulfonate cation-exchange or trimethylammonium anion-exchange ligands on porous PS-DVB beads. |
| Performance Range / Specifications | Particle sizes of approximately 10, 15 or 30 µm; dynamic capacities are about 60-80 mg/mL for lysozyme on cation exchangers and about 45-65 mg/mL for BSA on anion exchangers. |
| Storage Conditions | 20% ethanol at 4-25 °C |
| Package Specifications | 30 mL, 50 mL, 100 mL, 300 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L |
| Product Form | High-resolution strong ion-exchange chromatography medium |
| Key Features | 10, 15 and 30 µm particle options High resolution with low backpressure Strong cation and anion exchange chemistries Pressure tolerance up to 8.0 MPa |
| Product Type | High-resolution ion-exchange chromatography medium |
| Function / Efficacy | Charge-based high-resolution purification of biomolecules. |
| Chromatography Mode | Strong cation-exchange or strong anion-exchange chromatography |
| Matrix | Monodisperse, highly crosslinked porous polystyrene-divinylbenzene (PS-DVB) |
| Particle Size | Approximately 10, 15 or 30 µm |
| Ligand / Bonded Phase | Sulfonate or trimethylammonium |
| Dynamic Binding Capacity | Approximately 80, 80 or 60 mg/mL for lysozyme on the cation exchangers; approximately 65, 55 or 45 mg/mL for BSA on the anion exchangers |
| Ligand Density | Approximately 0.19-0.28 meq/mL, depending on particle size and ligand |
| Maximum Operating Pressure | 2.0-8.0 MPa, depending on particle size and ligand |
| CIP / Sanitization Conditions | 0.5 M NaOH for the anion exchangers; 1 M NaOH for the cation exchangers |
| Recommended Flow Rate | 100-750 cm/h for 10 µm, 150-800 cm/h for 15 µm, and 250-1000 cm/h for 30 µm media |
| pH Stability | pH 2-12 |
| Chemical Stability | Compatible with common buffers, 1 M acetic acid, 1 M NaOH, 1 M HCl, alcohols, acetonitrile, DMSO, DMF, 1% SDS, 6 M guanidine hydrochloride and 8 M urea, with solvent coverage varying by medium. Avoid strong oxidants, oxidizing acids, strong reducing agents, acetone and benzyl alcohol. |
| Operating Temperature | 4-30 °C |
For research use only, not for clinical use.
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