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| Product Name | Acid Phosphatase Activity Assay Kit |
| Catalog No. | ETR-HMM-0076 |
| Description | A colorimetric assay kit for measuring acid phosphatase activity in biological samples using p-nitrophenyl phosphate as substrate at acidic pH. Acid phosphatase is a key lysosomal enzyme and biomarker for prostate cancer (prostatic acid phosphatase). |
| Intended Use | Quantitative measurement of acid phosphatase activity in serum, plasma, prostate tissue extracts, seminal fluid, and cell lysates. |
| Principle / Technology | Acid phosphatase catalyzes the hydrolysis of p-nitrophenyl phosphate to p-nitrophenol at pH 4.8–5.5; the enzymatic reaction is terminated by raising the pH with sodium hydroxide, which simultaneously develops the yellow color of p-nitrophenol. |
| Detection Method | Endpoint colorimetric measurement at 405–410 nm |
| Sample Type | Human serum, seminal plasma, tissue homogenates, cell lysates |
| Performance Range / Specifications | Linear range: 0.2–100 U/L acid phosphatase activity |
| Sensitivity / LOD | ≤0.1 U/L detection limit |
| Specificity | Detects all acid phosphatase isoenzymes (prostatic, lysosomal, erythrocytic, and platelet); tartrate inhibition can differentiate prostatic from other acid phosphatase isoforms |
| Reaction Conditions / Protocol | Add 100 μL of pre-warmed substrate solution to 20 μL of sample, incubate at 37°C for 30 minutes, add 100 μL of stop/color development reagent, read absorbance at 405 nm |
| Components / Formulation | Citrate buffer (pH 4.8) with pNPP substrate, tartrate inhibitor (for prostatic ACP differentiation), acid phosphatase positive control, stop reagent (NaOH), standard p-nitrophenol solution |
| Storage Conditions | -20°C for substrate reagents; buffer at 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 500 tests |
| Product Form | Liquid reagents |
| Quality Control | Each lot validated with purified prostatic acid phosphatase standard; tartrate inhibition verified; standard curve R² >0.99 |
| Key Features | Inclusion of tartrate inhibitor enables differentiation of prostatic acid phosphatase for prostate cancer biomarker assessment; simple colorimetric detection compatible with standard equipment |
For research use only, not for clinical use.
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