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| Product Name | α-Glucosidase Inhibitor Screening Assay Kit |
| Catalog No. | ETR-HMM-0080 |
| Description | A colorimetric screening assay kit for identifying and characterizing alpha-glucosidase inhibitors from natural product extracts and synthetic compound libraries. The assay utilizes a chromogenic nitrophenyl-glucopyranoside substrate that releases a yellow product upon enzymatic hydrolysis by alpha-glucosidase. The kit includes acarbose as a reference inhibitor for screening validation and IC₅₀ determination. |
| Intended Use | High-throughput screening of alpha-glucosidase inhibitory compounds for antidiabetic drug discovery and functional food research. |
| Principle / Technology | Enzymatic hydrolysis of p-nitrophenyl-α-D-glucopyranoside by α-glucosidase producing colored p-nitrophenol |
| Detection Method | Colorimetric (absorbance at 405 nm) |
| Sample Type | Purified compounds, natural product extracts, herbal medicine fractions |
| Performance Range / Specifications | Screening throughput: compatible with 96-well and 384-well formats; reaction time: 30 min |
| Sensitivity / LOD | Z′-factor: ≥0.7 in 96-well format (excellent screening quality) |
| Specificity | Enzyme selective for α-1,4-glucosidic linkages; no significant p-nitrophenyl-β-D-glucopyranoside hydrolysis |
| Reaction Conditions / Protocol | Pre-incubate test compound with enzyme 5 min at 37 °C; add substrate; incubate 30 min; read at 405 nm |
| Components / Formulation | α-Glucosidase (from Saccharomyces cerevisiae, lyophilized), pNPG substrate, acarbose reference inhibitor (1 mM), assay buffer (phosphate, pH 6.8), DMSO for compound solubilization |
| Storage Conditions | –20 °C for enzyme; 2–8 °C for substrate and inhibitor; room temperature for buffer |
| Shelf Life | 12 months for enzyme (desiccated); 24 months for other reagents |
| Package Specifications | 1000 tests (96-well format) |
| Product Form | Lyophilized enzyme; liquid reagents |
| Quality Control | Each enzyme lot tested with acarbose control; IC₅₀ for acarbose within literature reference range |
| Key Features | Pre-validated positive control (acarbose) provided for direct IC₅₀ comparison in every screening plate |
| Purity | Reagent grade; substrate purity ≥98% by HPLC where applicable |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold packs for enzyme components; ambient for buffers |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
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