| Product Name | Trypsase (TRY) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0008 |
| Description | Trypsin (Trypsase) is a serine protease that selectively hydrolyzes peptide chains composed of lysine or arginine carboxyl groups in denatured proteins. As an important digestive enzyme, it has widespread applications in food, pharmaceuticals, leather, textiles, cosmetics, livestock, healthcare, and biological research. |
| Application | Trypsin can catalyze the hydrolysis of the ester bond in N-benzoyl-L-arginine ethyl ester (BAEE), producing N-benzoyl-L-arginine (BA). The product has a characteristic absorption peak at 253 nm, and the activity of trypsin can be characterized by changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 100 Samples |
| Matching | 96-well plate |
| Detection Time | 4 h (100 Samples) |
| Detection Method | Folin method |
| Spectral Parameters | 253 nm |
| Signal Response | Incremental |
| Notes | If the A2 measurement is greater than 1.5 or ∆A is greater than 0.15, it is recommended to dilute the crude enzyme solution appropriately before performing the measurement again; if ∆A is less than 0.01, it is recommended to increase the sample volume appropriately or extend the enzymatic reaction time before performing the measurement again, and make corresponding adjustments during calculation; Accurately measure the absorbance values at 10 s and 70 s to ensure the accuracy and reproducibility of the experimental results; |
For research use only, not for clinical use.
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