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| Product Name | One-Step Multiplex RT-PCR Kit, Low-Background Version (DNA and RNA Pathogen Co-Detection) |
| Catalog No. | REKI-0108 |
| Description | A one-step multiplex RT-PCR reagent that carries reverse transcription and hot-start multiplex amplification through in a single tube, built for amplicon library construction in pathogen sequencing. Panels scale from 1 to 2000-plex, amplicons between 100 and 500 bp amplify across a 25 to 75 percent GC window, and uniformity, specificity and sensitivity all stay high across panel sizes. Because reverse transcription and multiplex amplification share one tube, RNA and DNA pathogens are co-detected without separate handling, and the chemistry extends beyond the standard range into several-thousand-plex amplicon capture. |
| Intended Use | Amplicon library construction for pathogen sequencing, syndromic co-detection of DNA and RNA pathogens, respiratory and blood culture pathogen panels. |
| Principle / Technology | Reverse transcription and hot-start multiplex PCR proceed sequentially in one tube; panel primers determine targets while the premix supplies enzyme, buffer and dNTPs. |
| Performance Range / Specifications | Panel depth 1 to 2000-plex; amplicons 100 to 500 bp; GC coverage 25 to 75 percent; high uniformity and sensitivity. |
| Specificity | Built-in hot-start chemistry; single-tube workflow reduces cross-contamination risk. |
| Reaction Conditions / Protocol | Add template and panel primers to the premix, run reverse transcription followed by multiplex amplification, then continue library finishing. |
| Components / Formulation | One-step RT-mPCR premix with hot-start polymerase, reverse transcriptase, reaction buffer, dNTPs. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 24 T, 48 T, 96 T, 1000 T |
| Product Form | Liquid premix |
| Key Features | True one-tube RT and amplification; low background across panel sizes; broad fragment flexibility. |
| Compatibility | Pathogen tNGS workflows on short-read sequencing platforms |
For research use only, not for clinical use.
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