Universal-ROX qPCR Library Quantification Kit for Illumina Libraries

Name: Universal-ROX qPCR Library Quantification Kit for Illumina Libraries
Price: Contact Alta DiagnoTech for price USD
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Cat.No: NG-QCY-0047 Datasheet

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Product Details Related Products

Product Name	Universal-ROX qPCR Library Quantification Kit for Illumina Libraries
Catalog No.	NG-QCY-0047
Description	A one-tube dye-based qPCR system for absolute quantification of Illumina-compatible sequencing libraries. Amplification reagents, primers and universal passive-reference dye are premixed to reduce setup steps and support fast cycling.
Intended Use	absolute concentration measurement of Illumina-compatible sequencing libraries and assessment of library contamination
Principle / Technology	Standard-curve-based absolute quantification using hot-start dye qPCR
Detection Method	Real-time fluorescence qPCR
Sample Type	Illumina-compatible sequencing libraries
Performance Range / Specifications	500 reactions; supports standard and fast qPCR programs
Sensitivity / LOD	High-sensitivity qPCR quantification across the supplied standard range
Specificity	Library-specific amplification depends on compatibility between the kit primers and library adapter sequences.
Reaction Conditions / Protocol	Add standards or diluted libraries to the premixed qPCR reagent, run a compatible program and calculate concentration from the standard curve.
Components / Formulation	Universal-ROX hot-start dye qPCR premix containing amplification reagents and primers, plus DNA standards
Storage Conditions	Store at −30 to −15°C.
Package Specifications	500 reactions
Product Form	Multi-component fluorescence quantification kit
Quality Control	Standards and detection reagents are functionally tested for quantitative performance.
Key Features	One-tube premix; universal passive-reference formulation; broad instrument compatibility; high specificity and amplification efficiency; fast workflow.
Shipping Conditions	Ship at 0°C or below.
Compatibility	Illumina-compatible library types and common real-time PCR instruments.
Batch-to-Batch Consistency	Quantitative performance and standard response are controlled across production batches.

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For research use only, not for clinical use.