Globin mRNA Depletion Probe Kit for Common Species

Name: Globin mRNA Depletion Probe Kit for Common Species
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Cat.No: NG-QCY-0016 Datasheet

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Product Name	Globin mRNA Depletion Probe Kit for Common Species
Catalog No.	NG-QCY-0016
Description	A probe-based depletion kit for removing abundant globin mRNA from blood-derived total RNA of commonly studied mammalian species.
Intended Use	mRNA and noncoding RNA analysis from blood total RNA of human, rat, mouse, Chinese hamster and macaque species.
Principle / Technology	Globin-specific probes hybridize to target transcripts, followed by RNase H digestion and DNase I treatment. The kit can be combined with a compatible rRNA depletion workflow.
Detection Method	Downstream RNA-seq library preparation and sequencing.
Sample Type	Intact or partially degraded blood total RNA.
Performance Range / Specifications	Input range 0.01–1 µg total RNA; 6, 24 or 96 reactions; approximately 1-hour workflow.
Sensitivity / LOD	Reported residual globin mRNA below 1%.
Specificity	Targets major alpha- and beta-globin transcripts while retaining other mRNA and noncoding RNA.
Reaction Conditions / Protocol	Combine with a compatible rRNA depletion workflow when required, hybridize the globin probes, perform RNase H and DNase I digestion, and purify the depleted RNA.
Components / Formulation	Globin-targeting depletion probes and compatible RNase H/DNase I depletion reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	6 reactions; 24 reactions; 96 reactions.
Product Form	Globin mRNA depletion probe kit.
Quality Control	Residual globin mRNA, species compatibility and downstream transcriptome sequencing quality are evaluated.
Key Features	Common-species coverage; below 1% residual globin mRNA; approximately 1-hour workflow; compatible with partially degraded RNA.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Designed for use with a compatible rRNA depletion system before RNA-seq library preparation.
Application Notes / Precautions	Confirm probe coverage for the target species before processing valuable samples.

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For research use only, not for clinical use.