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| Product Name | Rapid DNA Ligation Module for Dual-Platform Library Construction |
| Catalog No. | REKI-0114 |
| Description | A ligation module engineered around a proprietary rapid T4 DNA ligase for library construction on both major short-read sequencing platforms. The enzyme joins adapters for mainstream high-throughput sequencing workflows onto double-stranded fragments whether the ends are blunt or carry a 3-prime dA overhang, with high ligation efficiency in a simple, automation-ready reaction. Validated end to end with companion end-repair and amplification modules through sequencing on both platforms, the module keeps adapter join rates high while shortening bench time. |
| Intended Use | Adapter ligation in DNA library construction for mainstream high-throughput sequencing workflows; automated library preparation; blunt-ended and A-tailed fragment adapter joining. |
| Principle / Technology | The rapid T4 DNA ligase catalyses phosphodiester bond formation between adapter and insert ends, tolerating both blunt and single-base A-overhang substrates. |
| Performance Range / Specifications | High ligation efficiency on blunt and A-tailed ends; validated with companion end repair and amplification modules by sequencing on both platforms. |
| Reaction Conditions / Protocol | Combine end-prepared DNA, adapters and ligation mix, incubate under the rapid programme, then purify the ligation product. |
| Components / Formulation | Rapid T4 DNA ligase, ligation buffer, reaction enhancers. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 24 T, 96 T |
| Product Form | Liquid reagents |
| Key Features | Proprietary rapid ligase; single reaction covers blunt and A-tailed substrates; automation-compatible setup. |
| Compatibility | mainstream high-throughput sequencing platforms; pairs with companion end-repair and amplification modules |
For research use only, not for clinical use.
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