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| Product Name | Uracil-Tolerant High-Fidelity DNA Polymerase 2x Premix for Methylation and FFPE Library Amplification |
| Catalog No. | REKI-0104 |
| Description | A ready-to-use 2x premix containing a uracil-tolerant high-fidelity DNA polymerase, dNTPs and a buffer optimised for sequencing library amplification. The enzyme reads through uracil-containing templates that defeat conventional proofreading polymerases, so bisulfite-converted and enzymatically converted methylation libraries amplify with full sensitivity. Damaged FFPE DNA benefits from the same tolerance. Carry-over amplicons containing dUTP can be degraded before a run, keeping aerosol contamination in check, and the chemistry also supports uracil-excision based cloning. Only primers and template are added, which trims bench time and improves reproducibility. |
| Intended Use | Methylation library amplification, multiplex PCR on converted templates, amplification of damaged FFPE DNA, prevention of amplicon aerosol contamination, uracil-excision cloning. |
| Principle / Technology | The uracil-tolerant polymerase extends primers across dU-containing strands without stalling, while dUTP-carrying carry-over products from previous runs are selectively removed prior to amplification. |
| Performance Range / Specifications | High sensitivity and specificity on converted templates; retains fidelity on damaged FFPE DNA. |
| Specificity | Reads through U-containing templates; compatible with carry-over control workflows. |
| Reaction Conditions / Protocol | Add primers and template to the 2x premix and run the recommended cycling programme. |
| Components / Formulation | Uracil-tolerant high-fidelity DNA polymerase, dNTP mix, optimised amplification buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 1 mL, 5 mL, 25 mL |
| Product Form | Liquid premix |
| Key Features | Ready-to-use premix reduces pipetting steps; strong batch-to-batch consistency; suits methylation and FFPE workflows alike. |
| Concentration | 2x premix |
| Compatibility | High-throughput sequencing library amplification workflows |
For research use only, not for clinical use.
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