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| Product Name | High-Efficiency DNA Library Preparation Kit |
| Catalog No. | NG-QCY-0011 |
| Description | A high-efficiency DNA library preparation kit for converting fragmented double-stranded DNA into sequencing-ready libraries for major short-read platforms. |
| Intended Use | Whole-genome sequencing, whole-exome sequencing, targeted capture sequencing and metagenomic sequencing. |
| Principle / Technology | Optimized end repair, adapter ligation and optional high-fidelity library amplification convert fragmented double-stranded DNA into platform-compatible libraries. |
| Detection Method | Downstream next-generation sequencing and library quality control. |
| Sample Type | Fragmented double-stranded DNA. |
| Performance Range / Specifications | Input range 0.1–500 ng; 24 or 96 reactions; supports PCR and PCR-free library workflows; reported library conversion approached 90%. |
| Sensitivity / LOD | Supports low-input fragmented DNA down to 0.1 ng. |
| Specificity | High-fidelity amplification and optimized ligation improve library uniformity and low-frequency variant detection. |
| Reaction Conditions / Protocol | Perform end preparation, adapter ligation, cleanup and optional library amplification using platform-compatible adapters and indexes. |
| Components / Formulation | End-preparation, adapter-ligation and library-amplification reagents for fragmented double-stranded DNA. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 24 reactions; 96 reactions. |
| Product Form | Multi-component DNA library preparation kit. |
| Quality Control | Library conversion, yield, uniformity, high-depth sequencing performance and low-frequency variant detection are evaluated. |
| Key Features | Broad 0.1–500 ng input range; high ligation efficiency; PCR and PCR-free compatibility; high-fidelity amplification; strong library uniformity. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with Illumina and MGI short-read sequencing platforms and targeted capture workflows. |
| Application Notes / Precautions | Select adapters, index primers and amplification cycles according to the sequencing platform and input amount. |
For research use only, not for clinical use.
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