Universal Circularization Kit for MGI Libraries

Name: Universal Circularization Kit for MGI Libraries
Price: Contact Alta DiagnoTech for price USD
Availability: InStock

Cat.No: NG-QCY-0055 Datasheet

Quantities:

- +

Add To Cart

Product Details Related Products

Product Name	Universal Circularization Kit for MGI Libraries
Catalog No.	NG-QCY-0055
Description	A modular circularization system for converting linear MGI-compatible libraries prepared with single- or dual-barcode adapters into single-stranded circular DNA libraries.
Intended Use	Circularization of single-barcode and dual-barcode dsDNA libraries for MGI sequencing.
Principle / Technology	Splint-assisted ligation circularizes library molecules, followed by digestion of remaining linear DNA.
Detection Method	MGI sequencing after DNB generation.
Sample Type	Linear dsDNA libraries carrying compatible single- or dual-barcode adapters.
Performance Range / Specifications	Circularization time as short as 1 hour; compatible input range approximately 0.1-2 pmol; 16 or 48 reactions.
Specificity	Optimized for MGI-compatible adapter structures and both single- and dual-barcode libraries.
Reaction Conditions / Protocol	Combine the linear library with circularization reagents, complete ligation, digest residual linear DNA and proceed to DNB preparation.
Components / Formulation	Universal splint-assisted circularization and linear-DNA digestion reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	16 reactions; 48 reactions.
Product Form	Multi-component library circularization kit.
Quality Control	Circularization efficiency and downstream DNB generation are functionally verified.
Key Features	Single- and dual-barcode compatibility; approximately 1-hour circularization; high effective circularization rate; broad template compatibility.
Shipping Conditions	Ship at 0°C or below.
Compatibility	MGI-compatible linear dsDNA libraries prepared with single- or dual-barcode adapters.
Application Notes / Precautions	Accurately quantify linear library input and confirm fragment-size distribution before circularization.
Batch-to-Batch Consistency	Circularization and DNB-yield performance are controlled across batches.

Cat.No	Product Name	Price
NG-QCY-0004	Small RNA Index Primer Kit	Add To Cart
NG-QCY-0012	Universal Enzymatic Fragmentation DNA Library Preparation Kit	Add To Cart
NG-QCY-0053	96-Plex Single-Index Adapter Plate for MGI Libraries	Add To Cart
NG-QCY-0026	Viral RNA Multiplex Amplicon Library Preparation Kit	Add To Cart
NG-QCY-0002	Library Preparation NGS Kit	Add To Cart
NG-QCY-0036	DNA Repair and A-Tailing Module for Nanopore Sequencing V2	Add To Cart
NG-QCY-0050	Unique Dual Index Primers for Illumina Tagmentation Libraries	Add To Cart
NG-QCY-0021	Long-Range Pathogen Multiplex PCR Master Mix	Add To Cart
NG-QCY-0027	Rapid Hi-C Library Preparation Kit	Add To Cart
NG-QCY-0024	Pathogen Multiplex PCR Mix for DNA and RNA	Add To Cart

For research use only, not for clinical use.