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| Product Name | Tyrode's Solution, Calcium-Free |
| Catalog No. | RERE-0443 |
| Description | Removing calcium from the bathing fluid is a standard maneuver when an experiment calls for the abolition of calcium dependent contractions, junctional processes or signaling steps, and this calcium-free Tyrode solution provides the medium for it. Sodium chloride, sodium dihydrogen phosphate and glucose remain in the recipe while the calcium chloride of the standard formulation is left out entirely, and HEPES is again excluded. It serves the same perfusion and rinsing duties as the parent fluid, keeping excised preparations viable minus the divalent cation. |
| Intended Use | Perfusion and rinsing of excised preparations under calcium-free conditions while maintaining physiological function. |
| Principle / Technology | The standard Tyrode salt balance is preserved except that calcium chloride is omitted, allowing calcium dependent processes in the tissue to be terminated or studied by exclusion. |
| Specificity | Contains sodium chloride, sodium dihydrogen phosphate and glucose; calcium omitted; HEPES-free. |
| Reaction Conditions / Protocol | Substitute directly for standard Tyrode solution whenever the protocol requires a calcium-free bathing or perfusing medium. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 500 mL |
| Key Features | Calcium withdrawn from the classical recipe; phosphate buffered and glucose fed; pairs with the standard Tyrode grade. |
For research use only, not for clinical use.
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