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| Product Name | Leupeptin Hemisulfate, Ultra-pure |
| Catalog No. | RERE-0035 |
| Description | Leupeptin is a reversible, competitive inhibitor of serine and thiol proteases, effectively blocking plasmin, trypsin, proteinase K, kallikrein, papain, calpain and cathepsins B, H and L, while leaving pepsin, cathepsin D, thrombin and alpha-chymotrypsin unaffected. It is widely used to protect target proteins during isolation from tissues and other samples, and can afterwards be removed by dialysis. The hemisulfate salt is the most widely commercialised form; this product is supplied as ultra-pure hemisulfate powder. |
| Intended Use | Protection of target proteins during isolation from tissue and other biological samples; removal by dialysis after extraction |
| Principle / Technology | Reversible competitive binding to the active site of serine and thiol proteases |
| Detection Method | Plasmin, trypsin, proteinase K, kallikrein, papain, calpain and cathepsins B, H and L |
| Sample Type | Tissue and cell extracts prepared for protein isolation |
| Performance Range / Specifications | Effective working range of 10 to 100 micromolar in extraction buffers |
| Specificity | Inhibits serine and thiol proteases; no activity against pepsin, cathepsin D, thrombin or alpha-chymotrypsin |
| Reaction Conditions / Protocol | Prepare a 10 mM aqueous stock; use at 10 to 100 micromolar in the extraction buffer |
| Components / Formulation | Leupeptin hemisulfate powder, 5 mg or 10 x 5 mg vials |
| Storage Conditions | -25 to -15°C for 24 months as supplied; 10 mM stock keeps 1 week at 4°C; aliquots keep at least 6 months at -20°C |
| Shelf Life | 24 months |
| Package Specifications | 5 mg; 10 x 5 mg |
| Product Form | White amorphous powder |
| Quality Control | Ultra-pure grade verified on the lot record; peptide identity confirmed |
| Key Features | Broad serine and thiol protease coverage; reversible inhibition removable by dialysis; ultra-pure hemisulfate salt; aliquot-friendly packaging |
| Purity | Ultra-pure |
| Concentration | 10 mM storage solution in water |
| Activity / Unit Definition | Reversible competitive protease inhibition |
| Molecular Weight | 475.59 |
| Source / Origin | Chemical or fermentation-derived peptide, supplied as hemisulfate |
| Shipping Conditions | Ambient temperature with cooling as required |
| Expiration Date / Stability | 24 months frozen as powder; working solutions of 10 to 100 micromolar stable only for hours and should be kept on ice |
| Regulatory / Compliance | For research use only |
| Compatibility | Compatible with protein isolation workflows; removable by dialysis before downstream assays |
| Application Notes / Precautions | Peptide sequence Ac-Leu-Leu-Arg-al; prepare working solutions fresh and avoid repeated freeze-thaw of stocks |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction and isolation with preserved proteolytic integrity |
For research use only, not for clinical use.
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