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| Product Name | HEPES-Potassium Acetate Lysis Buffer, Sterile |
| Catalog No. | RERE-0406 |
| Description | Protoplast work lives or dies by osmotic balance, and this lysis buffer, also called simply HEPES lysis buffer, protects isolated plant protoplasts with 0.1 M sorbitol while HEPES holds the pH and potassium acetate supplies the ionic background. EDTA rounds out the recipe by sequestering divalent cations that would otherwise license nuclease activity. Filter-sterilised and kept cold, it supports the fractionation of protoplast components with cell contents intact. |
| Intended Use | Disruption and component separation of plant protoplasts under osmotically protective conditions. |
| Principle / Technology | Sorbitol balances osmotic pressure, HEPES buffers pH and EDTA chelates cations to restrain nucleases. |
| Specificity | Composed of HEPES, potassium acetate, 0.1 M sorbitol and EDTA; filter-sterilised. |
| Reaction Conditions / Protocol | Use as supplied for protoplast lysis and fractionation under aseptic conditions. |
| Storage Conditions | 2-8°C; 3 months. |
| Package Specifications | 100 mL |
| Key Features | Osmoprotected lysis chemistry; EDTA-guarded against nuclease damage; short three-month shelf life reflects its fresh formulation. |
For research use only, not for clinical use.
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