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| Product Name | E-64, Cysteine Protease Inhibitor |
| Catalog No. | RERE-0038 |
| Description | E-64 is an irreversible, potent and highly selective inhibitor of cysteine proteases and of calpain activation. It penetrates cells and tissues with low toxicity, which is why it is widely used in living-system work as well as straightforward extraction protection. Serine proteases are not affected, with a single documented exception among trypsin-like enzymes. The compound dissolves in water and stays stable across pH 2 to 10, though it breaks down in ammonia or hydrochloric acid. |
| Intended Use | Inhibition of cysteine proteases and calpain activation during protein extraction; suitable for cell-permeable applications in living systems |
| Principle / Technology | Irreversible covalent modification of the active-site cysteine residue of target proteases |
| Detection Method | Cysteine proteases and calpain activation pathways |
| Sample Type | Cell and tissue extracts; living-cell systems where membrane permeability is required |
| Performance Range / Specifications | Potent inhibition at 1 to 10 micromolar working concentration; stable across pH 2 to 10 |
| Specificity | Highly selective for cysteine proteases; serine proteases unaffected |
| Reaction Conditions / Protocol | Dissolve in water and dose at 1 to 10 micromolar in the extraction or culture system |
| Components / Formulation | E-64 powder, 5 mg per vial |
| Storage Conditions | 2 to 8°C |
| Package Specifications | 5 mg |
| Product Form | White powder |
| Quality Control | Purity at least 99% confirmed on the lot record |
| Key Features | Irreversible and highly selective; cell and tissue permeable with low toxicity; water-soluble; stable across a wide pH window |
| Purity | At least 99% |
| Concentration | Water-soluble; dose at 1 to 10 micromolar |
| Activity / Unit Definition | Irreversible cysteine protease inhibition |
| Molecular Weight | 357.41 |
| Source / Origin | Chemical or fermentation-derived compound |
| pH Range / Optimal pH | Stable across pH 2 to 10 |
| Shipping Conditions | Wet ice |
| Expiration Date / Stability | Stable across pH 2 to 10; decomposes in ammonia or hydrochloric acid |
| Regulatory / Compliance | For research use only |
| Compatibility | Combines with serine and aspartyl protease inhibitors for broad-coverage extraction cocktails |
| Application Notes / Precautions | Avoid exposure to ammonia or strong acid; the membrane-permeable ester analogue E-64d is available where better cell entry is needed |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction protected from cysteine protease activity; calpain activation studies |
For research use only, not for clinical use.
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