| Product Name | TUNEL Apoptosis Detection Reagent (Colorimetric Method) |
| Catalog No. | CCAT-HMM-0033 |
| Description | During apoptosis, certain DNA endonucleases are activated, which cleave genomic DNA between nucleosomes. When DNA is extracted from apoptotic cells and subjected to electrophoresis, a DNA ladder with a size of 180–200 bp can be observed. When genomic DNA breaks, the exposed 3'-OH group can be labeled with biotin-labeled dUTP (Biotin-dUTP) under the catalysis of terminal deoxynucleotidyl transferase (TdT). which then binds to horseradish peroxidase (HRP)-labeled streptavidin (Streptavidin-HRP). Finally, under the catalysis of HRP, DAB staining is used to visualize apoptotic cells, enabling their detection via a standard optical microscope. This is the principle behind the TUNEL (TdT-mediated dUTP Nick-End Labeling) assay for detecting apoptosis. |
| Application | This product provides a highly sensitive, rapid, and convenient method for detecting apoptosis. |
| Applicable Instruments | Optical microscope |
| Materials to Bring | PBS or HBSS for washing cells; appropriate mounting medium such as anti-fluorescence quenching mounting medium; 4% paraformaldehyde or immunostaining fixative (P0098) for fixation; PBS containing 0.3% Triton X-100 or immunostaining permeabilization medium; hydrogen peroxide. |
| Storage | Store at -20°C away from light. |
| Features | High sensitivity: Extremely low background staining and strong positive staining enable detection of apoptosis at the single-cell level. Additionally, since DNA fragmentation occurs early in the apoptosis process, early-stage apoptosis can be detected. Good specificity: TUNEL staining typically labels apoptotic cells more effectively than necrotic cells. Rapid: The process can be completed in approximately 2–3 hours. Wide application range: It can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspension cells. |
| Notes | If used for paraffin section detection, proteinase K and xylene must be provided by the user. DAB is harmful to humans. Please handle with care and take effective precautions to avoid direct contact with the body or inhalation. For your safety and health, please wear a lab coat and disposable gloves when handling. |
For research use only, not for clinical use.
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