| Product Name | Annexin V-FITC Apoptosis Detection Reagent |
| Catalog No. | CCAT-HMM-0023 |
| Description | Annexin V selectively binds to phosphatidylserine (PS). PS is primarily distributed on the inner side of the cell membrane, i.e., the side adjacent to the cytoplasm. During the early stages of apoptosis, various cell types translocate phosphatidylserine to the cell surface, i.e., the outer side of the cell membrane. Once exposed on the cell surface, phosphatidylserine promotes coagulation and inflammatory responses. However, when Annexin V binds to phosphatidylserine translocated to the cell surface, it can block the procoagulant and proinflammatory activities of phosphatidylserine. Annexin V labeled with the green fluorescent probe FITC, known as Annexin V-FITC, can be used to detect the outward flipping of phosphatidylserine—a key feature of apoptosis—in a simple and direct manner using flow cytometry or fluorescence microscopy. |
| Application | This product uses FITC-labeled recombinant human Annexin V to detect phosphatidylserine, which appears on the cell membrane surface during apoptosis. |
| Applicable Instruments | Flow cytometer, fluorescence microscope, or other fluorescence detection equipment |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; sterile ddH₂O |
| Storage | Store at 4°C away from light. |
| Shelf Life | 6 months |
| Notes | Although testing has shown that repeated freeze-thaw cycles (5 times) do not significantly affect the detection performance of Annexin V-FITC, it is recommended to store the product at 4°C within 3-6 months to achieve optimal results, and to avoid repeated freeze-thaw cycles whenever possible. Bacterial or fungal contamination can severely impair detection performance. It is advisable to perform the assay promptly after staining, as prolonged incubation may lead to an increase in the number of apoptotic or necrotic cells. If trypsin was used during cell collection, care must be taken to remove any residual trypsin. Residual trypsin can digest and degrade Annexin V-FITC, leading to staining failure. Fluorescent substances are prone to quenching. When performing fluorescence observations, minimize observation time and ensure light-protected storage during handling and storage. When using Annexin V-FITC for flow cytometry analysis, if an excessive number of PI false-positive cells are observed during Annexin V-FITC staining alone, and adjustments to settings and parameters fail to improve the situation, dilute Annexin V-FITC with PBS at a ratio of 3–10 times before testing. This typically effectively reduces the number of false-positive necrotic cells. For your safety and health, please wear a lab coat and disposable gloves during handling. |
For research use only, not for clinical use.
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