| Product Name | Annexin V-mCherry Apoptosis Detection Reagent |
| Catalog No. | CCAT-HMM-0026 |
| Description | Annexins are a class of calcium-dependent phospholipid-binding proteins widely distributed in the cytoplasm of eukaryotic cells, participating in intracellular signal transduction. However, only Annexin V has been reported to regulate the activity of certain PKCs. Annexin V selectively binds to phosphatidylserine (PS). PS is primarily distributed on the inner side of the cell membrane, adjacent to the cytoplasm. During the early stages of apoptosis, different cell types translocate phosphatidylserine to the cell surface, i.e., the outer side of the cell membrane. Once exposed to the cell surface, phosphatidylserine promotes coagulation and inflammatory responses. However, when Annexin V binds to phosphatidylserine that has been inverted to the cell surface, it can block the procoagulant and proinflammatory activities of phosphatidylserine. Annexin V-mCherry has a narrower excitation spectrum compared to phycoerythrin (PE)-labeled Annexin V and does not produce red fluorescence around 495 nm like PE, making it highly suitable for co-detection with other green fluorescent markers. During flow cytometry analysis, virtually no compensation is required. |
| Application | This product uses red fluorescent protein mCherry-labeled recombinant human Annexin V to detect phosphatidylserine that appears on the cell membrane surface during apoptosis. |
| Spectral Parameters | Ex/Em (nm) = 587/610 |
| Applicable Instruments | Flow cytometer, fluorescence microscope, or other fluorescence detection equipment |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; sterile ddH₂O |
| Storage | Store at -20°C away from light. |
| Shelf Life | 1 year |
| Notes | If there is bacterial or fungal contamination, it will severely affect the detection results. After staining, testing should be conducted as soon as possible. Prolonged exposure may lead to an increase in the number of apoptotic or necrotic cells. If trypsin was used during cell collection, care must be taken to remove any residual trypsin. Residual trypsin can digest and degrade Annexin V-mCherry, ultimately resulting in staining failure. Fluorescent substances are prone to quenching. When performing fluorescence observations, minimize observation time and ensure light-protected storage during handling and storage. For your safety and health, please wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
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