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| Product Name | Cell Cycle Detection Reagent (DNA Content Detection, PI Red Fluorescence) |
| Catalog No. | CCAT-HMM-0004 |
| Description | This product employs DNA content analysis, using flow cytometry to measure DNA content for cell cycle analysis and determine the percentage of cell populations at different stages. The principle behind this method is that DNA within cells can specifically bind to certain fluorescent dyes (such as PI, DAPI, etc.), which are then used to label and detect DNA content. The intensity of the bound fluorescent dye is directly proportional to DNA content, and by measuring the fluorescence intensity using a flow cytometer, the proportion of cells in each phase of the cell cycle can be determined. |
| Application | This product is suitable for detecting the cell cycle of adherent cells and suspension cells. The fluorescence intensity of the PI signal is directly proportional to DNA content. The histogram of DNA content can estimate the number of cells in the G1/G0, S, and G2/M phases. |
| Applicable Instruments | Flow cytometer |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; anhydrous ethanol; sterile ddH₂O |
| Storage | RNase A solution: Store at -20°C; PI solution: Store at 2–8°C away from light. |
| Shelf Life | 1 year |
| Notes | 1. Fluorescent substances are prone to quenching. When conducting fluorescence observations, minimize observation time as much as possible. Additionally, take care to avoid exposure to light during handling and storage. 2. PI solution is irritating to the human body. Handle with care and take appropriate precautions to avoid direct contact with the skin or inhalation. 3. For your safety and health, please wear a lab coat and disposable gloves when handling. |
For research use only, not for clinical use.
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