- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Propidium Iodide Cell Cycle Staining Kit with RNase |
| Catalog No. | CCAT-HMM-0042 |
| Description | A DNA content analysis kit that uses propidium iodide, a stoichiometric fluorescent DNA intercalator, combined with RNase treatment to eliminate RNA interference. The stained nuclei are analyzed by flow cytometry to resolve the distribution of cells across the G0/G1, S, and G2/M phases of the cell cycle. |
| Intended Use | Cell cycle phase distribution analysis in cancer biology research, cell synchronization experiments, anti-proliferative drug mechanism studies, and evaluation of cell cycle checkpoint activation. |
| Principle / Technology | Propidium iodide intercalates between DNA base pairs in a stoichiometric manner, binding in direct proportion to cellular DNA content; RNase A digestion removes double-stranded RNA that would otherwise contribute to the PI fluorescence signal; cells with 2N DNA content represent G0/G1, those with 4N content represent G2/M, and intermediate values correspond to S phase. |
| Detection Method | Flow cytometry with 488 nm laser excitation, fluorescence collected in FL2/PE channel (575/26 nm bandpass filter) |
| Sample Type | Single-cell suspensions of mammalian cells from culture, dissociated tissue, or blood; nuclei preparations from fresh or frozen solid tumors |
| Performance Range / Specifications | Cell cycle histograms with coefficient of variation (CV) ≤3% for G0/G1 peak using optimally prepared cells; linear DNA content resolution from 0.5C to 8C |
| Sensitivity / LOD | Discrimination of G0/G1 and G2/M peaks with DNA content ratios of 1.95–2.05; resolution adequate for detection of <5% aneuploid population |
| Specificity | PI fluorescence intensity is proportional to DNA content and independent of chromatin condensation state; RNase treatment eliminates RNA background signal; fixation/permeabilization renders the assay specific for total DNA rather than mitochondrial or bacterial DNA |
| Reaction Conditions / Protocol | Harvest cells (1 × 10^6), wash with cold PBS, fix with 70% ice-cold ethanol dropwise while vortexing; incubate at 4°C for at least 2 hours or overnight; wash twice with PBS; resuspend in PI/RNase staining buffer; incubate 30 minutes at 37°C in the dark; analyze by flow cytometry acquiring at least 10,000 events per sample |
| Components / Formulation | Propidium Iodide solution (1 mg/mL in water), RNase A (DNase-free, 20 mg/mL stock or lyophilized), staining buffer with Triton X-100, sodium citrate, and PBS; ethanol for fixation (not included) |
| Storage Conditions | -20°C protected from light; PI stock stable for 12 months; RNase A stable at -20°C for 12 months; staining buffer at 2–8°C |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 100 tests, 500 tests (flow cytometry format) |
| Product Form | Individual concentrated reagent solutions requiring dilution before use |
| Quality Control | Each lot examined by flow cytometry using chicken erythrocyte nuclei (CEN) and calf thymocyte nuclei (CTN) reference standards; linearity and doublet discrimination resolution verified; RNase free of DNase activity |
| Key Features | Robust and widely cited DNA content analysis method; compatible with most flow cytometers using 488 nm excitation; doublet discrimination gating eliminates aggregated cell interference; suitable for ethanol-fixed cells stored for up to 2 weeks |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
