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| Product Name | Magnesium (Mg) Colorimetric Assay Kit, 96 T (80 Samples), 48 T (32 Samples) |
| Catalog No. | ETK-YJL-0008 |
| Size | 96 T (80 samples), 48 T (32 samples) |
| Sample Type | Serum (plasma) |
| Detection Instrument | Microplate reader (520-550 nm, optimal detection wavelength: 540 nm) |
| Research Field | Inorganic salt ions; Plant physiology series |
| Sensitivity | 0.18 mmol/L |
| Detection Range | 0.18-2.50 mmol/L |
| Accuracy | Inter-assay variation: 7.9% | Intra-assay variation: 5.1% |
| Sample Size | 2.5 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | Mg |
| Detection Time | 20 min |
Magnesium (Mg) is an indispensable inorganic cation in biological systems and holds a pivotal role in maintaining normal physiological functions across various organisms—making its accurate quantification critical for scientific research. As the 11th most abundant element in the human body, magnesium ions (Mg²⁺) are not only concentrated in bones, muscle cells, and soft tissues but also present in serum and red blood cells, serving as a core regulator of multiple biological processes.
In research contexts, understanding magnesium levels is essential for advancing studies in inorganic salt ions and plant physiology, two key fields where this assay kit is applied. For instance:
In animal and human biological research, Mg²⁺ acts as a cofactor for over 300 enzymes, driving reactions related to nucleic acid synthesis, protein metabolism, and ATP (adenosine triphosphate) utilization. It also modulates cardiac excitability, neuromuscular signal conduction, vasomotor contraction, and systemic energy balance—making serum/plasma magnesium measurement vital for studies on metabolic disorders, cardiovascular physiology, and neurological function.
In plant physiology research, magnesium is the central metallic ion in chlorophyll, the pigment responsible for capturing light energy during photosynthesis. It directly influences plant growth, nutrient uptake, and stress resistance (e.g., drought or salinity tolerance). Quantifying magnesium in plant-derived samples (when paired with appropriate sample pretreatment) helps researchers evaluate plant health, optimize fertilizer use, and explore mechanisms of stress adaptation.
Traditional methods for magnesium detection often suffer from limitations such as complex sample pretreatment, long assay times, or low sensitivity—barriers that hinder efficient, high-throughput research. The Magnesium (Mg) Colorimetric Assay Kit addresses these challenges by leveraging a reliable colorimetric principle, enabling rapid, accurate, and reproducible magnesium quantification in serum and plasma samples. This kit is specifically designed for research use only (not for clinical diagnostics), providing researchers with a robust tool to support studies in inorganic ion metabolism, plant physiology, and related interdisciplinary fields.
Broad Compatibility with Research Samples: Tailored for serum and plasma samples (common in animal/human physiological research), with potential adaptability for other sample types (e.g., plant extracts or cell lysates) following pre-test validation of dilution factors—expanding its utility across diverse research projects.
Optimized Detection Performance: Features a detection range of 0.18–2.50 mmol/L and a sensitivity of 0.18 mmol/L, which aligns with the physiological concentration range of magnesium in most biological samples. This ensures that both low and moderate magnesium levels can be accurately measured, avoiding false negatives or inaccurate quantitation.
Rapid Assay Workflow: Completes the entire detection process in just 20 minutes, significantly reducing experimental time compared to traditional methods (e.g., atomic absorption spectroscopy). This efficiency supports high-throughput sample analysis, ideal for labs handling large batches of samples.
Minimal Sample Requirement: Only 2.5 μL of sample is needed per assay, minimizing sample consumption—critical for precious or limited-volume samples (e.g., serum from small animal models or rare clinical research specimens).
Easy-to-Use Instrument Compatibility: Designed for use with a standard microplate reader (detection wavelength: 520–550 nm, optimal at 540 nm), a common piece of equipment in most research labs. No specialized or expensive instruments are required, lowering the barrier to adoption.
Stable Storage and Long Shelf Life: Can be stored at 2–8°C (protected from light) for up to 6 months, ensuring reagent stability and reducing the need for frequent reordering. This stability also guarantees consistent performance across multiple experiments over time.
High Accuracy and Reproducibility: Maintains strict quality control standards, with an inter-assay variation of only 7.9% and an intra-assay variation of 5.1%. These low coefficient of variation (CV) values ensure that results are consistent both within the same experiment (intra-assay) and across different experiments or operators (inter-assay)—a key requirement for reliable research data.
User-Friendly Operation: Requires no complex sample pretreatment (e.g., digestion or purification) for serum/plasma samples, simplifying the experimental workflow. Even researchers with limited experience in ion detection can follow the protocol easily, reducing the risk of human error and training costs.
Cost-Effective Sample Throughput: Available in two sizes (96 T for 80 samples, 48 T for 32 samples), allowing labs to choose the appropriate format based on their sample volume needs. This flexibility prevents waste of reagents and reduces per-sample testing costs, making it suitable for both small-scale pilot studies and large-scale research projects.
Research-Specific Design: Explicitly optimized for research use, with performance parameters tailored to the needs of inorganic salt ion and plant physiology studies. Unlike clinical-grade kits (which may have overly strict regulatory constraints), this kit balances sensitivity and practicality to support diverse research objectives.
Low Interference Risk: The colorimetric principle (based on specific complex formation between magnesium and reagents) minimizes interference from common substances in serum/plasma (e.g., other cations or proteins). This ensures that results reflect true magnesium concentrations, enhancing data reliability for downstream analysis.
For research use only, not for clinical use.
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