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| Product Name | TMK Buffer (Tris-MgCl2-KCl), pH 7.6 |
| Catalog No. | RERE-0407 |
| Description | Endoplasmic reticulum membranes demand a fractionation medium tuned to their chemistry, and TMK delivers it with Tris-HCl for pH control, magnesium chloride and potassium chloride for ionic strength. Sometimes described as Tris-MgCl2-KCl buffer, this preparation supports the isolation of ER fractions whose integrity depends on divalent cation management during homogenisation. It stores at room temperature, an unusual convenience among fractionation buffers. |
| Intended Use | Isolation of endoplasmic reticulum fractions from homogenised cells or tissues. |
| Principle / Technology | Tris buffering with controlled Mg2+ and K+ levels preserves ER membrane structure through fractionation. |
| Specificity | Composed of Tris-HCl, magnesium chloride and potassium chloride; pH 7.6. |
| Reaction Conditions / Protocol | Use as the homogenisation and suspension medium in ER isolation protocols. |
| Storage Conditions | Room temperature; 12 months. |
| Package Specifications | 100 mL |
| Key Features | Purpose-built ER isolation chemistry; stable at room temperature; simple three-component formula. |
For research use only, not for clinical use.
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