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TMK Buffer (Tris-MgCl2-KCl), pH 7.6

Cat.No: RERE-0407 Datasheet

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Product Name TMK Buffer (Tris-MgCl2-KCl), pH 7.6
Catalog No. RERE-0407
Description Endoplasmic reticulum membranes demand a fractionation medium tuned to their chemistry, and TMK delivers it with Tris-HCl for pH control, magnesium chloride and potassium chloride for ionic strength. Sometimes described as Tris-MgCl2-KCl buffer, this preparation supports the isolation of ER fractions whose integrity depends on divalent cation management during homogenisation. It stores at room temperature, an unusual convenience among fractionation buffers.
Intended Use Isolation of endoplasmic reticulum fractions from homogenised cells or tissues.
Principle / Technology Tris buffering with controlled Mg2+ and K+ levels preserves ER membrane structure through fractionation.
Specificity Composed of Tris-HCl, magnesium chloride and potassium chloride; pH 7.6.
Reaction Conditions / Protocol Use as the homogenisation and suspension medium in ER isolation protocols.
Storage Conditions Room temperature; 12 months.
Package Specifications 100 mL
Key Features Purpose-built ER isolation chemistry; stable at room temperature; simple three-component formula.

For research use only, not for clinical use.

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