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| Product Name | Tissue Homogenization Buffer, RIPA-Based with Protease/Phosphatase Inhibitor Cocktail |
| Catalog No. | ATR-SPS-0024 |
| Description | Ready-to-use RIPA-based homogenization buffer pre-supplemented with broad-spectrum protease and phosphatase inhibitor cocktail. Balanced ionic/non-ionic detergent combination efficiently solubilizes cytoplasmic, membrane, and nuclear proteins from mammalian tissues. Pre-added inhibitors eliminate preparation variability. |
| Intended Use | Total protein extraction from fresh/frozen mammalian tissues for Western blot, IP, ELISA, proteomics; phosphoprotein analysis; fibrous tissue lysis (heart, muscle, skin). |
| Principle / Technology | RIPA: sodium deoxycholate (ionic) solubilizes membrane proteins; NP-40 (non-ionic) extracts cytoplasmic proteins; SDS (low conc) aids nuclear extraction; comprehensive inhibitors block serine/threonine/cysteine/metallo-proteases and phosphatases |
| Detection Method | 500 µL per 20 mg tissue; homogenize on ice (30 sec, 2-3 pulses); incubate 30 min on ice; centrifuge 14,000×g 20 min at 4 °C; collect supernatant |
| Sample Type | Fresh/frozen tissues: liver, kidney, brain, heart, lung, spleen, muscle, adipose, skin, intestine, tumor biopsies; 10⁷ cells per 500 µL |
| Components / Formulation | Tris-HCl 50 mM, NaCl 150 mM, Na-deoxycholate 0.5%, NP-40 1%, SDS 0.1%, EDTA 1 mM, Na₃VO₄ 1 mM, NaF 5 mM, Na-pyrophosphate 5 mM, PMSF 1 mM, aprotinin/leupeptin/pepstatin A/bestatin/E-64 (2-5 µg/mL each), β-glycerophosphate 10 mM |
| Storage Conditions | -20 °C in single-use aliquots; inhibitors labile; protect from light |
| Shelf Life | 12 months at -20 °C; use within 24 hr after thawing |
| Package Specifications | 50 mL, 100 mL, 250 mL amber bottles; single-use aliquots available |
| Product Form | Clear to pale yellow liquid; may precipitate — warm to RT and vortex |
| Key Features | Ready-to-use — no inhibitor addition; comprehensive inhibitor coverage; optimized for fibrous tissues; EDTA-free version available; PMSF pre-dissolved |
| Purity | All inhibitors pharmaceutical/molecular biology grade; PMSF >95%; no detectable protease in blank |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | pH 7.4 ± 0.1 at 4 °C |
| Shipping Conditions | Cold pack -20 °C; dry ice for >48 hr transit; PMSF hydrolyzes in solution — temperature critical |
| Expiration Date / Stability | 12 months at -20 °C; use within 24 hr after thaw; PMSF half-life ~30 min at 25 °C; work on ice |
| Regulatory / Compliance | For laboratory and research use only; RUO; PMSF toxic — handle with gloves in fume hood; ISO 9001 |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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