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| Product Name | Tissue Dissociation Enzyme Cocktail, Collagenase-Based |
| Catalog No. | ATR-SPS-0009 |
| Description | An enzyme blend containing collagenase type I and II, dispase, and DNase I for the gentle enzymatic dissociation of solid tissues into viable single-cell suspensions for primary cell culture and single-cell analysis. |
| Intended Use | Dissociation of mouse and human tumor tissues, normal solid organs, and biopsy specimens into viable single-cell preparations for flow cytometry, scRNA-seq, and primary cell culture establishment. |
| Principle / Technology | Collagenase degrades extracellular matrix collagen fibers; dispase cleaves fibronectin and type IV collagen in basement membrane; DNase I reduces clumping by digesting DNA released from damaged cells. |
| Detection Method | Cell yield and viability by AO/PI staining, flow cytometric assessment of surface antigen preservation |
| Sample Type | Solid tumors, liver, lung, kidney, spleen, lymph nodes, skin biopsies |
| Performance Range / Specifications | Cell yield 10^6–10^7 cells per gram tissue; viability >85% |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Mince tissue into 1–2 mm pieces; add enzyme cocktail; incubate at 37°C with agitation for 30–60 minutes; pass through 70 µm strainer; wash cells. |
| Components / Formulation | Collagenase Type I, Collagenase Type II, Dispase, DNase I, in HBSS with calcium and magnesium |
| Storage Conditions | -20°C, protect from light, avoid freeze-thaw |
| Shelf Life | 12 months at -20°C |
| Package Specifications | Lyophilized, sufficient for 5 g or 10 g tissue |
| Product Form | Lyophilized powder, reconstitute in sterile HBSS |
| Quality Control | Enzyme activity verification, cell viability testing with reference mouse tumor, sterility |
| Key Features | Gentle dissociation preserves surface antigens; DNase prevents clumping; validated for tumor and normal tissue; compatible with 10x Genomics single-cell protocols |
For research use only, not for clinical use.
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