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| Product Name | Sucrose-Imidazole Solution, 2.25 M, pH 7.0 |
| Catalog No. | RERE-0419 |
| Description | At 2.25 M sucrose this is the densest formulation in the sucrose-imidazole family, delivering the bottom layer against which lighter yeast membranes float during gradient separation. The standard 25 mM imidazole and 2 mM EDTA chemistry holds pH 7.0 and suppresses enzymatic degradation respectively. Laboratories assembling complete yeast plasma membrane gradients draw on this grade together with its 0.4 M, 1.1 M and 1.65 M siblings. |
| Intended Use | Bottom, highest-density layer of sucrose gradients used to isolate yeast plasma membranes. |
| Principle / Technology | Maximal sucrose density creates the terminal gradient step that traps or retains the least buoyant material. |
| Specificity | 2.25 M sucrose; 2 mM EDTA; 25 mM imidazole; pH 7.0. |
| Reaction Conditions / Protocol | Use as the densest layer of the gradient according to the plasma membrane isolation protocol. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Densest grade of the series; EDTA protected; pairs with the lighter cushion solutions. |
For research use only, not for clinical use.
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