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| Product Name | Sucrose-Imidazole Solution, 1.65 M, pH 7.0 |
| Catalog No. | RERE-0418 |
| Description | Dense sucrose layers are the backbone of membrane gradient separations, and this 1.65 M sucrose-imidazole solution supplies one of the highest-density steps in the yeast plasma membrane series. Imidazole at 25 mM anchors pH 7.0 while 2 mM EDTA disarms metal-dependent enzymes that would otherwise degrade the preparation during the long centrifugation. It complements the 0.4 M, 1.1 M and 2.25 M grades that bracket the same gradient. |
| Intended Use | High-density cushion layer in yeast plasma membrane gradient fractionation. |
| Principle / Technology | The concentrated sucrose layer establishes the density step that resolves membranes during ultracentrifugation. |
| Specificity | 1.65 M sucrose; 2 mM EDTA; 25 mM imidazole; pH 7.0. |
| Reaction Conditions / Protocol | Layer beneath lighter fractions according to the gradient design of the plasma membrane protocol. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Near-highest density grade of the series; EDTA guarded; completes the multi-step gradient set. |
For research use only, not for clinical use.
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