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| Product Name | Sucrose-Imidazole Solution, 1.1 M, pH 7.0 |
| Catalog No. | RERE-0417 |
| Description | Gradient fractionation of yeast membranes relies on density steps, and this 1.1 M sucrose-imidazole solution supplies the dense layer beneath which lighter membranes float away from contaminants. Imidazole at 25 mM holds pH 7.0 and 2 mM EDTA suppresses metalloprotease and nuclease activity throughout the spin. It is the high-density partner of the 0.4 M grade in the same plasma membrane protocol. |
| Intended Use | Dense cushion and gradient medium in yeast plasma membrane isolation workflows. |
| Principle / Technology | A concentrated sucrose layer creates the density step that resolves membranes during centrifugation. |
| Specificity | 1.1 M sucrose; 2 mM EDTA; 25 mM imidazole; pH 7.0. |
| Reaction Conditions / Protocol | Layer or mix according to the gradient design of the plasma membrane isolation protocol. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | High-density 1.1 M grade; EDTA guarded; completes the two-solution gradient set for yeast membranes. |
For research use only, not for clinical use.
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