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| Product Name | Sucrose-Imidazole Solution, 0.4 M, pH 7.0 |
| Catalog No. | RERE-0416 |
| Description | Yeast plasma membrane isolation lives on osmotic cushioning, and this 0.4 M sucrose medium provides it alongside 25 mM imidazole for pH control and 2 mM EDTA to silence metal-dependent enzymes. The composition is tuned so that membranes fractionate cleanly while soluble contents wash away. It complements the denser 1.1 M grade used for gradient steps in the same workflow. |
| Intended Use | Isolation of plasma membrane fractions from yeast cells during organelle fractionation. |
| Principle / Technology | Sucrose cushions the membranes while imidazole buffers and EDTA restrains metalloenzyme activity. |
| Specificity | 0.4 M sucrose; 2 mM EDTA; 25 mM imidazole; pH 7.0. |
| Reaction Conditions / Protocol | Use as the suspension or gradient medium in yeast plasma membrane isolation protocols. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Isotonic cushioning grade; EDTA protected; pairs with the 1.1 M solution for gradient work. |
For research use only, not for clinical use.
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