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| Product Name | Succinate Assay Reagent |
| Catalog No. | CMTR-HMM-0031 |
| Description | Succinic acid (also known as succinic acid or amber acid) has the molecular formula C₄H₆O₄ and a molecular weight of 118.09. Succinic acid is a dicarboxylic acid that can undergo most typical reactions of dicarboxylic acids. Due to the presence of two active methylene groups in its molecule, it also exhibits several other important reaction characteristics, such as esterification, chlorination, and iodination. Succinic acid has very widespread applications in industries such as food, pharmaceuticals, chemicals, and environmental protection. |
| Application | This product is used to measure succinate levels in biological samples. |
| Principle | Succinic acid is utilized by succinyl-CoA synthetase to generate an intermediate, which undergoes a series of reactions to reduce the colorless probe into a colored product with strong absorbance at OD = 450 nm. |
| Synonyms | butanedioic acid; ethane-12 dicarboxylic acid; ethylenesuccinic acid; spirit of amber; succinate |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 30 min |
| Sample Type | Adherent cells, Suspension cells, Tissue |
| Detection Limit | 40 µM |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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