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| Product Name | Alpha Ketoglutarate (alpha KG) Assay Reagent |
| Catalog No. | CMTR-HMM-0038 |
| Description | Alpha-KG is an important nitrogen transport protein. As a key intermediate, it is one of the organic acids measured in newborns and can serve as an indicator of congenital metabolic defects. |
| Application | This product is used to measure alpha-KG levels in biological samples. |
| Principle | Alpha-ketoglutarate undergoes a transamination reaction with pyruvate, which is used to convert an almost colorless probe into a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product. |
| Synonyms | Alpha KG |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 30 min |
| Sample Type | Adherent cells, Suspension cells, Tissue |
| Detection Limit | 0.01 nmol/well |
| Standard Linear Range | 0.01 nmol/well - 10 nmol/well |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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