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| Product Name | ATP Bioluminescence Assay Kit (Luciferase-Based, Ultra-Sensitive) |
| Catalog No. | CMTR-HMM-0072 |
| Description | Ultra-sensitive bioluminescence ATP assay using recombinant firefly luciferase and D-luciferin. ATP-dependent light emission at 560 nm is directly proportional to ATP concentration over a 7-log range, detecting sub-picomole to nanomole ATP levels. Glow-type signal with >4 hour half-life supports batch plate processing in high-throughput formats. |
| Intended Use | Cellular ATP level measurement, mitochondrial function assessment, drug-induced mitochondrial toxicity screening, cell proliferation quantification, and bacterial contamination detection. |
| Principle / Technology | Firefly luciferase catalyzes: Luciferin + ATP + O₂ → Oxyluciferin + AMP + PPi + CO₂ + Light (560 nm); light intensity proportional to ATP |
| Detection Method | Bioluminescence; integration time 0.1-1.0 sec; glow half-life >4 hours |
| Sample Type | Cell lysates, tissue homogenates, bacterial cultures; ATP extraction with lysis buffer |
| Sensitivity / LOD | LOD 0.01 pmol ATP/well (~10 mammalian cells); dynamic range 0.01-10,000 pmol; 7-log linearity |
| Specificity | Highly specific for ATP; dATP cross-reactivity <1%; no significant signal from ADP, AMP, GTP, CTP |
| Reaction Conditions / Protocol | Lyse cells with ATP lysis buffer; add luciferase/luciferin reagent; mix; read luminescence |
| Components / Formulation | Recombinant firefly luciferase, D-luciferin, ATP standard (10 µmol), ATP lysis buffer (10×), assay buffer, ATP-free water |
| Storage Conditions | Store at -80 °C long-term; -20 °C up to 3 months; protect luciferin from light |
| Shelf Life | 12 months at -80 °C; 6 months at -20 °C |
| Package Specifications | 100 assays, 500 assays, 2,000 assays; HTS formats available |
| Product Form | Lyophilized enzyme and substrate; liquid buffers |
| Key Features | Sub-picomole sensitivity; 7-log dynamic range; >4 hour glow half-life; recombinant luciferase with enhanced thermostability; ATP-free water included |
| Purity | Luciferase >10¹⁰ RLU/mg; D-luciferin >99% HPLC; ATP standard >99% |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Dry ice -80 °C; do not thaw; luciferin moisture-sensitive |
| Expiration Date / Stability | 12 months at -80 °C; 6 months at -20 °C; reconstituted reagent 24 hr at 4 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
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