STM Solution (Sucrose-Tris-MgCl2), 0.25 M, pH 7.4, Sterile
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STM Solution (Sucrose-Tris-MgCl2), 0.25 M, pH 7.4, Sterile

Cat.No: RERE-0414 Datasheet

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Product Name STM Solution (Sucrose-Tris-MgCl2), 0.25 M, pH 7.4, Sterile
Catalog No. RERE-0414
Description Separating plant root tips or epidermal layers from intact tissue calls for an osmotic buffer that neither plasmolyses the cells nor invites their enzymes to run wild. This sterile 0.25 M formulation of sucrose-Tris-MgCl2 does both jobs, with the sugar balancing water potential, Tris-HCl anchoring pH 7.4 and magnesium chloride stabilising membranes. Filter-sterilised and frozen, it is the gentler sibling of the 2 M concentrate.
Intended Use Separation of plant root tip or epidermal cells for cytological and molecular analysis.
Principle / Technology Sucrose osmotic balance with Tris-MgCl2 ionic control preserves plant cell integrity during separation.
Specificity 0.25 M sucrose with Tris-HCl and magnesium chloride; pH 7.4; sterile, filter-sterilised.
Reaction Conditions / Protocol Use as the separation medium for root tip or epidermal tissue according to the protocol.
Storage Conditions -20°C; 12 months.
Package Specifications 100 mL
Key Features Isotonic 0.25 M grade for delicate separations; sterile; frozen storage keeps it ready.

For research use only, not for clinical use.

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