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| Product Name | ssDNA Fluorescence Quantitation Kit (1-200 ng) |
| Catalog No. | REKI-0097 |
| Description | A simple, sensitive fluorescence assay for single-stranded DNA covering a 1 to 200 ng linear window with detection down to 50 pg/uL. The kit contains dye reagent, buffer and ssDNA standards; working solution is prepared fresh, mixed with samples and read on a fluorometer or fluorescence plate reader. The chemistry holds up against proteins, salts and detergents, and suits quantification of oligonucleotides, primers, denatured DNA and single-stranded circular DNA in library construction pipelines. |
| Intended Use | Quantification of ssDNA libraries, circularisation products, oligonucleotides, primers and denatured DNA in NGS workflows. |
| Principle / Technology | A ssDNA-responsive fluorescent dye reports concentration across a defined linear window. |
| Sample Type | Single-stranded DNA, oligonucleotides, primers, denatured DNA, single-stranded circular DNA |
| Performance Range / Specifications | Linear 1 to 200 ng; detection limit 50 pg/uL; tolerant of protein, salt and detergent contaminants. |
| Specificity | Reported for ssDNA targets; dsDNA selectivity is not elevated. |
| Reaction Conditions / Protocol | Prepare working solution, mix with standards and samples, incubate and read fluorescence. |
| Components / Formulation | Fluorescence dye reagent, buffer, ssDNA standards. |
| Storage Conditions | 2 to 8 °C |
| Shelf Life | 1 year |
| Package Specifications | 100 T, 500 T |
| Product Form | Fluorescence assay kit |
| Key Features | Sensitive ssDNA window; contaminant tolerant; standards included. |
| Compatibility | Fluorometers and fluorescence plate readers |
| Readouts / Downstream Assays | ssDNA concentration for library workflows |
For research use only, not for clinical use.
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