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ssDNA Fluorescence Quantification Kit

Cat.No: NG-QCY-0046 Datasheet

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Product Details Related Products
Product Name ssDNA Fluorescence Quantification Kit
Catalog No. NG-QCY-0046
Description A fluorescence assay for sensitive quantification of single-stranded DNA. The reagent provides a linear response over the stated ssDNA range and tolerates salts, nucleotides, proteins, solvents and detergents.
Intended Use quantification of single-stranded DNA
Principle / Technology Fluorescent dye binding to single-stranded DNA
Detection Method Fluorometric measurement
Sample Type Single-stranded DNA samples
Performance Range / Specifications Total ssDNA range: 1-200 ng; sample concentration range: 50 pg/μL-200 ng/μL
Sensitivity / LOD Quantifies as little as 1 ng total ssDNA within the stated linear range
Specificity The assay detects ssDNA but is not more selective for ssDNA than for dsDNA; common contaminants are well tolerated.
Reaction Conditions / Protocol Prepare the assay working solution, add the ssDNA sample and measure fluorescence after equilibration.
Components / Formulation Fluorescence detection reagent, assay buffer and ssDNA standards
Storage Conditions Store at 2-8°C protected from light; store the higher-concentration standard at −30 to −15°C for long-term storage.
Package Specifications 100 assays; 500 assays
Product Form Multi-component fluorescence quantification kit
Quality Control Standards and detection reagents are functionally tested for quantitative performance.
Key Features Sensitive ssDNA measurement; wide concentration range; dye binding reaches saturation within approximately 2 minutes; strong contaminant tolerance.
Shipping Conditions Ship with cold packs.
Compatibility Qubit fluorometers and compatible fluorescence readers.
Batch-to-Batch Consistency Quantitative performance and standard response are controlled across production batches.

For research use only, not for clinical use.

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