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| Product Name | Sorbitol-Phosphate Solution, 1.2 M, pH 7.5 |
| Catalog No. | RERE-0423 |
| Description | Combining the osmotic muscle of 1.2 M sorbitol with phosphate buffering at pH 7.5 and a dose of EDTA, this solution is a complete isolation medium rather than a mere additive. The sugar alcohol protects fragile organelles, the phosphate pair stabilises pH and the chelator restrains nucleases that would otherwise fragment the very structures being purified. It pairs with the sterile version of the same formulation for contamination-sensitive work. |
| Intended Use | Isolation medium for nuclei and related organelles under osmotic and nuclease-protected conditions. |
| Principle / Technology | Sorbitol balances osmotic pressure while phosphate buffers and EDTA chelates cations needed by nucleases. |
| Specificity | 1.2 M sorbitol; composed with phosphate and EDTA; pH 7.5. |
| Reaction Conditions / Protocol | Use as the isolation medium according to the nuclei isolation protocol. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Three-function formulation in one solution; pH 7.5; matches the sterile grade in composition. |
For research use only, not for clinical use.
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