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| Product Name | Sodium Orthovanadate |
| Catalog No. | RERE-0041 |
| Description | Sodium orthovanadate is a competitive phosphatase inhibitor that blocks alkaline phosphatase, acid phosphatase and tyrosine phosphatases, and also inhibits ATPases such as the sodium-potassium pump. During protein extraction it keeps phosphorylation states intact, which is why it appears in RIPA-type lysis formulations. The inhibition is reversible on EDTA addition or dilution. The powder must be activated by depolymerisation to the monomeric form before use to achieve full phosphatase inhibitory activity. |
| Intended Use | Preservation of protein phosphorylation states during extraction from cells and tissues; general phosphatase inhibition |
| Principle / Technology | Competitive inhibition of phosphatase active sites; reversible on EDTA addition or dilution |
| Detection Method | Alkaline phosphatase, acid phosphatase, tyrosine phosphatases and ATPases such as Na+/K+ ATPase |
| Sample Type | Cell and tissue lysates where phosphorylation states matter |
| Performance Range / Specifications | Effective at 1 to 10 mM after activation to the monomeric form |
| Specificity | Broad phosphatase coverage with additional ATPase inhibition |
| Reaction Conditions / Protocol | Activate the powder by depolymerisation as described in the instructions, then prepare a 100 to 200 mM stock and dose at 1 to 10 mM |
| Components / Formulation | Sodium orthovanadate powder, 10 g per container |
| Storage Conditions | Room temperature as powder; 100 to 200 mM stock aliquots keep 12 months at -20°C |
| Package Specifications | 10 g |
| Product Form | White to off-white powder |
| Quality Control | Purity above 96% confirmed on the lot record; activation procedure supplied |
| Key Features | Broad phosphatase inhibition preserving phosphorylation; reversible with EDTA; large economical pack; activation protocol included |
| Purity | Above 96% |
| Concentration | Use at 1 to 10 mM; stocks of 100 to 200 mM in water |
| Activity / Unit Definition | Competitive phosphatase and ATPase inhibition |
| Molecular Weight | 183.91 |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | Stable dry at room temperature; frozen aliquoted stocks 12 months |
| Regulatory / Compliance | For research use only |
| Compatibility | Standard component of RIPA-type lysis workflows; pairs with protease inhibitors for complete protection |
| Application Notes / Precautions | Activation before first use is essential for full activity; reversible inhibition should be considered when interpreting phosphatase-dependent assays |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Phosphorylation state preservation in Western blotting and phosphoprotein analysis |
For research use only, not for clinical use.
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