Small RNA Library Prep Kit for Short-Read Sequencing Platforms
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Small RNA Library Prep Kit for Short-Read Sequencing Platforms

Cat.No: REKI-0076 Datasheet

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Product Name Small RNA Library Prep Kit for Short-Read Sequencing Platforms
Catalog No. REKI-0076
Description A dedicated kit for constructing small RNA sequencing libraries on short-read platforms. It handles small RNAs purified from animal or plant cells and tissues as well as total RNA preparations rich in exosomal small RNA. Gel-free magnetic-bead size selection removes the need for PAGE purification, cutting handling time while retaining short inserts, and the box includes every bead and adapter required apart from alcohol and the sample itself. Input ranges from 100 ng to 1 ug of total RNA, with demonstration data extending up to 4 ug.
Intended Use Small RNA expression profiling and functional studies in animals, plants and fungi; developmental biology; environmental adaptation and trait research; pharmacological intervention studies.
Principle / Technology Adapter-ligated small RNAs are reverse transcribed, PCR amplified and size selected on magnetic beads to build sequencing-ready small RNA libraries without gel recovery.
Sample Type Cells, tissues, exosomes, total RNA
Performance Range / Specifications Input 100 ng to 1 ug total RNA; gel-free bead-based size selection; compatibility with automated pipetting workstations.
Specificity Optimised for short-insert small RNA species including miRNA and other regulatory RNAs.
Reaction Conditions / Protocol Ligate adapters to small RNAs, reverse transcribe, amplify and select library size on magnetic beads.
Components / Formulation All beads and adapters required for library construction; user supplies only alcohol and sample.
Storage Conditions Box I and Box II at -25 to -15 °C; one dedicated component at -80 °C; Box III at 2 to 4 °C
Package Specifications 8 T, 24 T, 96 T
Product Form Liquid reagents in boxed modules
Key Features No gel recovery; single-box convenience; exosomal small RNA compatible; automation-friendly.
Starting Material / Tissue Site 100 ng to 1 ug total RNA input
Readouts / Downstream Assays Small RNA-seq expression libraries

For research use only, not for clinical use.

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