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| Product Name | Single-Stranded DNA Library Circularization Kit for MGI |
| Catalog No. | NG-QCY-0056 |
| Description | A modular kit for converting adapter-ligated PCR products into single-stranded circular DNA libraries for MGI sequencing platforms. |
| Intended Use | Preparation of single-stranded circular DNA libraries for MGI high-throughput sequencing. |
| Principle / Technology | Splint oligonucleotide-directed ligation circularizes library molecules, followed by enzymatic removal of residual linear DNA. |
| Detection Method | MGI sequencing after DNB generation. |
| Sample Type | Adapter-ligated PCR library products. |
| Performance Range / Specifications | Circularization time as short as 70 minutes; reported circularization efficiency up to 60%; 16 or 48 reactions. |
| Specificity | Optimized for compatible MGI library structures while supporting different library methods, inputs and template types. |
| Reaction Conditions / Protocol | Mix the library with splint oligonucleotide and ligation reagents, incubate for circularization, then digest remaining linear molecules. |
| Components / Formulation | Splint oligonucleotide, rapid splint buffer, rapid DNA ligase, digestion buffer and digestion enzyme. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 16 reactions; 48 reactions. |
| Product Form | Multi-component circularization kit. |
| Quality Control | Reagents are functionally tested for circularization yield, sequencing data quality and reproducibility. |
| Key Features | Rapid circularization; efficiency up to 60%; broad library-template compatibility; stable sequencing performance. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | MGI-compatible adapter-ligated PCR libraries. |
| Application Notes / Precautions | Use accurately quantified libraries with a suitable fragment-size profile and avoid repeated freeze-thaw cycles. |
| Batch-to-Batch Consistency | Circularization yield and downstream sequencing performance are controlled across batches. |
For research use only, not for clinical use.
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