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| Product Name | Single-Cell Whole-Genome Amplification Kit V2 |
| Catalog No. | NG-QCY-0032 |
| Description | A Phi29-based multiple-displacement amplification kit for high-coverage whole-genome amplification from single cells or ultra-low DNA inputs. |
| Intended Use | Whole-genome sequencing, whole-exome sequencing, copy-number variation analysis, microsatellite analysis, qPCR, microarray and preimplantation genetic testing research. |
| Principle / Technology | Phi29 DNA polymerase performs isothermal multiple-displacement amplification with strong strand displacement and 3′→5′ proofreading activity. |
| Detection Method | Downstream sequencing, PCR, qPCR, microarray or genotyping analysis. |
| Sample Type | Single cells and compatible low-input DNA samples. |
| Performance Range / Specifications | Reported genome coverage above 95%; amplified DNA products approximately 2–100 kb with an average length above 15 kb; 24 or 96 reactions. |
| Sensitivity / LOD | Designed for amplification from a single cell. |
| Specificity | High-fidelity Phi29 polymerase and isothermal amplification support broad genome coverage with reduced replication error. |
| Reaction Conditions / Protocol | Lyse the sample, prepare the isothermal amplification reaction and incubate under validated MDA conditions before enzyme inactivation and downstream analysis. |
| Components / Formulation | Single-cell lysis and Phi29 multiple-displacement amplification reagents. |
| Storage Conditions | For routine storage, keep at −30 to −15°C; for extended storage, keep at −85 to −65°C. |
| Package Specifications | 24 reactions; 96 reactions. |
| Product Form | Multi-component MDA whole-genome amplification kit. |
| Key Features | Above 95% reported coverage; long amplified products; strong strand displacement; proofreading activity; high amplification fidelity. |
| Shipping Conditions | Ship on dry ice. |
| Expiration Date / Stability | Long-term storage is specified at −85 to −65°C. |
| Compatibility | Suitable for WGS, WES, large-CNV analysis, microsatellite analysis, qPCR, microarray and related single-cell genome workflows. |
| Application Notes / Precautions | Prevent exogenous DNA contamination and avoid repeated freeze–thaw cycles of enzyme-containing components. |
For research use only, not for clinical use.
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