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| Product Name | Single-Cell Whole Genome Amplification Kit (MDA isothermal, Phi29-based) |
| Catalog No. | REKI-0080 |
| Description | A multiple displacement amplification kit that expands a single cell, or at least 1 ng of trace nucleic acid, into microgram quantities of genomic DNA through isothermal Phi29 polymerase reaction. Products span 2 to 100 kb with yields of at least 5 ug, and the high processivity of Phi29 combined with the kit formulation gives high genomic coverage with low amplification bias, supporting sequencing, large-insert CNV analysis, microsatellite analysis, qPCR and microarray readouts. |
| Intended Use | Whole genome amplification from single cells or trace nucleic acid; preimplantation genetic testing; large-insert copy number variation analysis; microsatellite and qPCR analysis; gene chip workflows; multi-omics extensions. |
| Principle / Technology | Isothermal strand-displacement amplification driven by Phi29 high-fidelity polymerase generates microgram DNA quantities at constant temperature. |
| Sample Type | Single cells, trace nucleic acid samples |
| Performance Range / Specifications | Input more than 1 cell or at least 1 ng nucleic acid; product 2 to 100 kb; yield at least 5 ug; high coverage with low bias. |
| Specificity | Phi29 high-fidelity polymerase chemistry; isothermal, no thermal cycler required. |
| Reaction Conditions / Protocol | Lyse the sample, start the isothermal amplification reaction and incubate; quantify and carry products to downstream assays. |
| Components / Formulation | Lysis reagent, Phi29 polymerase, amplification buffer, dNTP mix. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Boxed enzyme and buffer modules |
| Key Features | Isothermal workflow; microgram yields; low-bias high-coverage amplification. |
| Starting Material / Tissue Site | Single cell (more than 1 cell) or at least 1 ng nucleic acid |
| Readouts / Downstream Assays | Amplified genomic DNA for sequencing, CNV, qPCR and microarray assays |
For research use only, not for clinical use.
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