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| Product Name | Single-Cell Whole-Genome Amplification Kit |
| Catalog No. | NG-QCY-0033 |
| Description | A single-tube multiple-displacement amplification kit for rapid, high-coverage amplification of genomic DNA from single cells or low-input samples. |
| Intended Use | Whole-genome sequencing, whole-exome sequencing, large-copy-number analysis, microsatellite analysis, qPCR, microarray and preimplantation genetics research. |
| Principle / Technology | An engineered Phi29 DNA polymerase performs isothermal strand-displacement amplification with 3′→5′ proofreading activity. |
| Detection Method | Downstream sequencing, PCR, qPCR, microarray or genotyping analysis. |
| Sample Type | One to 1,000 animal or plant cells, bacteria, blastomeres, trophectoderm cells, sperm and other compatible low-input samples. |
| Performance Range / Specifications | Approximately 2-hour amplification; reported genome coverage above 95%; products approximately 2–100 kb with an average length above 20 kb; 24 or 96 reactions. |
| Sensitivity / LOD | Supports single-cell and other ultra-low-input samples. |
| Specificity | High-fidelity isothermal amplification supports broad genome representation and long DNA products. |
| Reaction Conditions / Protocol | Add the sample to the single-tube setup, complete lysis and isothermal amplification, then stop the reaction and use the product directly or after cleanup. |
| Components / Formulation | Whole-genome amplification enzyme mix, master buffer, sample-preparation buffer, stop solution, DTT, cell storage buffer and nuclease-free water. |
| Storage Conditions | Store at −30 to −15°C. For extended storage, keep enzyme-containing components below −70°C. |
| Package Specifications | 24 reactions; 96 reactions. |
| Product Form | Multi-component MDA whole-genome amplification kit. |
| Key Features | Single-tube setup in under 10 minutes; approximately 2-hour amplification; above 95% reported coverage; long amplified products; no mandatory post-amplification purification. |
| Compatibility | Suitable for WGS, WES, CNV analysis above approximately 1 Mb, microsatellite analysis, qPCR, microarray and preimplantation genetics research. |
| Application Notes / Precautions | Use strict clean-area procedures to prevent trace DNA contamination. Select downstream cleanup according to the application. |
For research use only, not for clinical use.
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