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| Product Name | Single Cell Suspension Preparation Reagent Kit (Enzymatic Tissue Dissociation) |
| Catalog No. | ATR-SPS-0019 |
| Description | A tissue dissociation kit containing a blend of purified collagenase and neutral protease enzymes optimized for gentle dissociation of solid tissues into viable single cell suspensions. The enzyme mixture preserves cell surface epitopes better than trypsin-based dissociation, making it suitable for downstream flow cytometry, single-cell RNA sequencing, and primary cell culture. The kit includes optimized digestion buffer and stop solution. |
| Intended Use | Enzymatic dissociation of solid tissue specimens into viable single cell suspensions for cytometry and single-cell analysis. |
| Principle / Technology | Collagenase and neutral protease-mediated extracellular matrix digestion with epitope preservation |
| Detection Method | Trypan blue viability counting; flow cytometry viability dye staining |
| Sample Type | Fresh solid tissue specimens: tumor biopsies, spleen, lymph node, lung, liver, and other parenchymal organs |
| Performance Range / Specifications | Typical cell yield: 1–5 × 10⁶ viable cells per 100 mg tissue; viability ≥85% |
| Sensitivity / LOD | Effective dissociation of tissues as small as 10 mg (needle biopsy scale) |
| Specificity | Broad tissue compatibility; collagenase blend digests collagens I–V |
| Reaction Conditions / Protocol | Mince tissue to 1–2 mm pieces; incubate in digestion medium 30–60 min at 37 °C with gentle agitation; filter through 70 µm strainer; wash cells; resuspend in appropriate buffer |
| Components / Formulation | Collagenase blend (Type I, II, IV lyophilized), neutral protease (lyophilized), digestion buffer (HBSS with calcium and magnesium), stop solution (FBS-containing medium), 70 µm cell strainers |
| Storage Conditions | Lyophilized enzymes: –20 °C; buffers: 2–8 °C or room temperature |
| Shelf Life | 12 months for enzymes; 18 months for buffers |
| Package Specifications | 10 tissue dissociations (up to 1 g each) per kit |
| Product Form | Lyophilized enzymes; liquid buffers |
| Quality Control | Viability and cell surface marker retention validated on mouse spleen and human tumor tissue |
| Key Features | Collagenase-based dissociation preserves labile surface markers that are lost with trypsin-based protocols |
| Purity | DNase/RNase free; sterile-filtered where applicable |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient for buffers; cold packs for enzyme components |
| Expiration Date / Stability | 12–18 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 or ISO 13485 quality system |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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