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Single-Cell DNA Library Preparation Kit

Cat.No: NG-QCY-0030 Datasheet

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Product Details Related Products
Product Name Single-Cell DNA Library Preparation Kit
Catalog No. NG-QCY-0030
Description A single-cell DNA library preparation kit that generates sequencing-ready libraries without a separate whole-genome pre-amplification step.
Intended Use Single-cell genome sequencing, low-input DNA sequencing, copy-number variation analysis and SNP analysis.
Principle / Technology A one-step low-bias library construction process converts cellular DNA directly into sequencing libraries, reducing amplification distortion and adapter-dimer formation.
Detection Method Illumina next-generation sequencing.
Sample Type One to 60 cells or equivalent ultra-low-input DNA.
Performance Range / Specifications Input range 1–60 cells; 24 or 96 reactions; designed for high coverage uniformity and sequence fidelity.
Sensitivity / LOD Supports library preparation from a single cell.
Specificity Direct low-input library construction limits pre-amplification bias and supports consistent genomic representation.
Reaction Conditions / Protocol Add the cell or low-input DNA sample to the library reaction, complete the one-step library construction workflow, then purify, quantify and sequence.
Components / Formulation Single-cell lysis and direct DNA library preparation reagents.
Storage Conditions Store at −30 to −15°C.
Package Specifications 24 reactions; 96 reactions.
Product Form Multi-component single-cell DNA library preparation kit.
Key Features Single-cell input; no separate WGA pre-amplification; low bias; high uniformity and fidelity; minimal adapter dimers; supports CNV and SNP analysis.
Shipping Conditions Ship at or below 0°C.
Compatibility Compatible with Illumina sequencing platforms.
Application Notes / Precautions Cell handling, lysis efficiency and contamination control are critical for single-cell library quality.

For research use only, not for clinical use.

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