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| Product Name | Single-Cell and Low-Input Whole Genome Amplification Kit (1-10 cells) |
| Catalog No. | REKI-0079 |
| Description | A whole genome amplification kit that starts from a single cell or trace DNA and finishes in roughly 160 minutes. The six-component system covers cell lysis buffer, lysis enzyme, pre-amplification buffer and enzyme, and amplification buffer and enzyme, producing 2 to 5 ug of amplified DNA with fragment sizes of 200 to 1500 bp. Products feed directly into enzymatic fragmentation library kits for downstream copy number analysis, SNP genotyping, qPCR and microarray workflows. |
| Intended Use | Copy number variation and SNP biomarker studies; preimplantation embryo testing and genome selection; transgenic animal genotyping; pollen and microbial genotyping; metagenomic detection of low-abundance bacteria. |
| Principle / Technology | Trace genomic DNA undergoes lysis followed by two-stage pre-amplification and amplification to microgram quantities of library-ready DNA. |
| Sample Type | Single cells, embryos, oocytes, pollen, microorganisms, cell-free DNA |
| Performance Range / Specifications | Input 1 to 10 cells or 10 to 50 pg DNA; product 200 to 1500 bp; 2 to 5 ug yield; about 160 min total. |
| Specificity | High amplification uniformity across the genome for downstream CNV and SNP calls. |
| Reaction Conditions / Protocol | Lyse cells, pre-amplify, then amplify to bulk quantities; proceed to library construction or genotyping assays. |
| Components / Formulation | Cell lysis buffer, cell lysis enzyme, pre-amplification buffer, pre-amplification enzyme, amplification buffer, amplification enzyme. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 24 reactions, 96 reactions |
| Product Form | Boxed enzyme and buffer modules |
| Key Features | Direct sample input; rapid 160-minute workflow; consistent amplification uniformity validated by QC. |
| Compatibility | Feeds enzymatic fragmentation library kits and standard genotyping assays |
| Starting Material / Tissue Site | 1-10 cells or 10-50 pg genomic DNA |
| Readouts / Downstream Assays | Amplified genomic DNA for CNV, SNP, qPCR and microarray analysis |
For research use only, not for clinical use.
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