Single-Cell and Low-Input Whole Genome Amplification Kit (1-10 cells)
Research
Online Inquiry

Single-Cell and Low-Input Whole Genome Amplification Kit (1-10 cells)

Cat.No: REKI-0079 Datasheet

Quantities:
- +
Product Details Related Products
Product Name Single-Cell and Low-Input Whole Genome Amplification Kit (1-10 cells)
Catalog No. REKI-0079
Description A whole genome amplification kit that starts from a single cell or trace DNA and finishes in roughly 160 minutes. The six-component system covers cell lysis buffer, lysis enzyme, pre-amplification buffer and enzyme, and amplification buffer and enzyme, producing 2 to 5 ug of amplified DNA with fragment sizes of 200 to 1500 bp. Products feed directly into enzymatic fragmentation library kits for downstream copy number analysis, SNP genotyping, qPCR and microarray workflows.
Intended Use Copy number variation and SNP biomarker studies; preimplantation embryo testing and genome selection; transgenic animal genotyping; pollen and microbial genotyping; metagenomic detection of low-abundance bacteria.
Principle / Technology Trace genomic DNA undergoes lysis followed by two-stage pre-amplification and amplification to microgram quantities of library-ready DNA.
Sample Type Single cells, embryos, oocytes, pollen, microorganisms, cell-free DNA
Performance Range / Specifications Input 1 to 10 cells or 10 to 50 pg DNA; product 200 to 1500 bp; 2 to 5 ug yield; about 160 min total.
Specificity High amplification uniformity across the genome for downstream CNV and SNP calls.
Reaction Conditions / Protocol Lyse cells, pre-amplify, then amplify to bulk quantities; proceed to library construction or genotyping assays.
Components / Formulation Cell lysis buffer, cell lysis enzyme, pre-amplification buffer, pre-amplification enzyme, amplification buffer, amplification enzyme.
Storage Conditions -25 to -15 °C
Package Specifications 24 reactions, 96 reactions
Product Form Boxed enzyme and buffer modules
Key Features Direct sample input; rapid 160-minute workflow; consistent amplification uniformity validated by QC.
Compatibility Feeds enzymatic fragmentation library kits and standard genotyping assays
Starting Material / Tissue Site 1-10 cells or 10-50 pg genomic DNA
Readouts / Downstream Assays Amplified genomic DNA for CNV, SNP, qPCR and microarray analysis

For research use only, not for clinical use.

0
0

There is no product in your cart.