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| Product Name | Single-Cell and Low-Input Full-Length RNA Library Prep Kit (enzyme-fragmentation) |
| Catalog No. | REKI-0078 |
| Description | A complete kit that takes 1 to 1000 cells, or 10 pg to 10 ng of total RNA, through full-length cDNA synthesis and enzyme-fragmentation library construction on both major short-read platforms. Reverse transcription uses template switching to capture complete transcripts while preserving the 5-prime and 3-prime untranslated regions, a coverage advantage over fragmentation-first workflows, at the cost of additional universal adapter and poly(A) sequence within reads. High-quality fragmentation enzymes and a streamlined workflow keep handling short and reproducible. |
| Intended Use | Single-cell transcriptome library construction; low-input RNA sequencing; gene expression and splicing analysis; fusion transcript detection. |
| Principle / Technology | Template-switching reverse transcription generates full-length cDNA that is then fragmented by enzymes, adapter ligated and amplified into sequencing libraries. |
| Sample Type | Single cells, total RNA |
| Performance Range / Specifications | Input 1 to 1000 cells or 10 pg to 10 ng RNA; 96-well and 384-well plate compatible; extraction-free entry. |
| Specificity | Retains 5-prime and 3-prime untranslated regions; adds universal adapter and poly(A) tail sequences. |
| Reaction Conditions / Protocol | Reverse transcribe with template switching, amplify full-length cDNA, fragment enzymatically, ligate adapters and amplify libraries. |
| Components / Formulation | Reverse transcription reagents and enzymatic fragmentation library construction reagents. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Boxed liquid reagent modules |
| Key Features | UTR-preserving coverage; dual-platform compatibility; extraction-free; simplified enzymatic fragmentation. |
| Compatibility | Compatible with mainstream short-read sequencing platforms |
| Starting Material / Tissue Site | 1-1000 cells or 10 pg to 10 ng total RNA |
| Readouts / Downstream Assays | Full-length transcript expression libraries |
For research use only, not for clinical use.
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