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| Product Name | Single-Cell and Low-Input Full-Length cDNA Synthesis and Amplification Module (1-1000 cells) |
| Catalog No. | REKI-0077 |
| Description | A module for synthesising full-length cDNA from as few as 1 to 1000 intact cells, or from 10 pg to 10 ng of total RNA, without any nucleic acid extraction step. Oligo(dT) priming combined with the template-switching activity of a engineered reverse transcriptase adds a universal adapter sequence at the cDNA end during reverse transcription, and PCR with that adapter enriches full-length transcripts. Reactions run in 96-well or 384-well plates with volumes of 5 uL or less and typically yield 2 to 20 ng of cDNA per reaction, feeding directly into tagmentation-based expression library kits. |
| Intended Use | Single-cell transcriptome library construction; gene expression differential analysis; alternative splicing studies; fusion gene detection; single B cell and molecular barcode workflows. |
| Principle / Technology | Oligo(dT)-primed reverse transcription with template switching appends a universal adapter to full-length cDNA, which then drives PCR enrichment of complete transcripts. |
| Sample Type | Single cells, total RNA |
| Performance Range / Specifications | Input 1 to 1000 cells or 10 pg to 10 ng total RNA; sample volume up to 5 uL; 2 to 20 ng cDNA per reaction; 96-well and 384-well plate compatible. |
| Specificity | Full-length transcript coverage; direct cell input without extraction. |
| Reaction Conditions / Protocol | Lyse cells, perform template-switching reverse transcription, amplify full-length cDNA by adapter-primed PCR. |
| Components / Formulation | Lysis and reverse transcription reagents, amplification reagent. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Liquid enzyme and buffer modules |
| Key Features | No extraction step; plates directly into expression library kits; suited to single B cell and barcode-tagged workflows. |
| Compatibility | Downstream connection to tagmentation-based expression library kits; 96/384-well plates |
| Starting Material / Tissue Site | 1-1000 cells or 10 pg to 10 ng total RNA |
| Readouts / Downstream Assays | Full-length cDNA for expression, splicing and fusion transcript analysis |
For research use only, not for clinical use.
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